This antibody gave a positive signal in both Human and Rat Testis tissue lysates.
This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The whole cell lysates in lanes 3-6 are used as negative controls for Spata18.
Western blot - Anti-Spata18 antibody (ab107702)
All lanes : Anti-Spata18 antibody (ab107702) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Human testis tissue lysate - total protein (ab30257) Lane 2 : Testis (Rat) Tissue Lysate Lane 3 : Human testis tissue lysate - total protein (ab30257) with Immunizing peptide at 1 µg/ml Lane 4 : Testis (Rat) Tissue Lysate with Immunizing peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Exposure time: 2 minutes
Abcam recommends using milk as the blocking agent (3%).
IHC image of Spata18 staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107702, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.