概述

  • 产品名称Anti-Spata18抗体
    参阅全部 Spata18 一抗
  • 描述
    兔多克隆抗体to Spata18
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Rabbit, Horse, Pig, Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Spata18.

  • 阳性对照
    • This antibody gave a positive signal in both Human and Rat Testis tissue lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal testis.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab107702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 61 kDa). Abcam recommends using milk as the blocking agent (3%).
IHC-P Use a concentration of 0.5 µg/ml.

靶标

  • 功能Potential role in spermatogenesis, especially in cell differentiation from late elongate spematids to mature spermatozoa.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1700067I02Rik antibody
    • FLJ32906 antibody
    • MGC93900 antibody
    • Mieap antibody
    • mitochondria eating protein antibody
    • SPATA18 antibody
    • Spermatogenesis associated 18 antibody
    • spermatogenesis associated 18 homolog (rat) antibody
    • Spermatogenesis associated protein 18 antibody
    • Spermatogenesis-associated protein 18 antibody
    • SPETEX1 antibody
    • SPT18_HUMAN antibody
    see all

Anti-Spata18 antibody 图像

  • All lanes : Anti-Spata18 antibody (ab107702) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Testis (Rat) Tissue Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • All lanes : Anti-Spata18 antibody (ab107702) at 1 µg/ml (Milk blocking - 3%)

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Testis (Rat) Tissue Lysate
    Lane 3 : Human testis tissue lysate - total protein (ab30257) with Immunizing peptide at 1 µg/ml
    Lane 4 : Testis (Rat) Tissue Lysate with Immunizing peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa


    Exposure time : 2 minutes
  • IHC image of Spata18 staining in Human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107702, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Spata18 antibody (ab107702)参考文献

ab107702 has not yet been referenced specifically in any publications.

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