概述

  • 产品名称Anti-SPATA13抗体
    参阅全部 SPATA13 一抗
  • 描述
    兔多克隆抗体to SPATA13
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    antigen: NQKKLAMLNA QKAGHGKSKG YNRCPVAPPH QGLHPIHQRH ITMPTSVPQQ QVFGLAEPKR KSSLFWHTFN RLT, corresponding to amino acids 576-648 of Human SPATA13 Isoforms 1,2,3 and 5 (Q96N96). This is partially absent in Isoform 4.

  • 阳性对照
    • RT-4 or U-251 MG lysate. Human bronchus tissue. Human A-431 cells

性能

应用

Our Abpromise guarantee covers the use of ab122701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

靶标

  • 功能Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
  • 组织特异性Expressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors.
  • 序列相似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • 结构域The C-terminal tail is required for its GEF activity.
  • 细胞定位Cytoplasm. Cell projection > filopodium. Cell projection > lamellipodium. Cell projection > ruffle membrane. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APC-stimulated guanine nucleotide exchange factor 2 antibody
    • ARHGEF29 antibody
    • Asef2 antibody
    • FLJ31208 antibody
    • RP11-307N16.3 antibody
    • Spata13 antibody
    • Spermatogenesis-associated protein 13 antibody
    • SPT13_HUMAN antibody
    see all

Anti-SPATA13 antibody 图像

  • All lanes : Anti-SPATA13 antibody (ab122701) at 1/250 dilution

    Lane 1 : RT-4 lysate
    Lane 2 : U251-MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Secondary
    Secondary antibody conjugated to horseradish peroxidase at 1/3000 dilution
  • ab122701, at 1/50 dilution, staining SPATA13 in Paraffin-embedded Human bronchus tissue by Immunohistochemistry
  • Immunofluorescent staining of Human cell line A-431 using ab122701 at 4µg/ml. Antibody staining is shown in green.

Anti-SPATA13 antibody (ab122701)参考文献

ab122701 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122701.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"