概述

  • 产品名称Anti-SPATA13抗体
    参阅全部 SPATA13 一抗
  • 描述
    兔多克隆抗体to SPATA13
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: ALRPAEWGTL DGSDLEDTDD AFQRSTHRSR SLRRAYGLGR ICLLDAPQNH ATPTIATGQV PAVCEILVRD PENNSMGYRR SKSTDNLAFL KKSSFKRKST SNLADLRTAH of Human SPATA13

  • 阳性对照
    • Human duodenum tissue; U 251 MG cells.

性能

应用

Our Abpromise guarantee covers the use of ab122627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.

靶标

  • 功能Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
  • 组织特异性Expressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors.
  • 序列相似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • 结构域The C-terminal tail is required for its GEF activity.
  • 细胞定位Cytoplasm. Cell projection > filopodium. Cell projection > lamellipodium. Cell projection > ruffle membrane. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APC-stimulated guanine nucleotide exchange factor 2 antibody
    • ARHGEF29 antibody
    • Asef2 antibody
    • FLJ31208 antibody
    • RP11-307N16.3 antibody
    • Spata13 antibody
    • Spermatogenesis-associated protein 13 antibody
    • SPT13_HUMAN antibody
    see all

Anti-SPATA13 antibody 图像

  • ab122627 at 1/200 dilution staining SPATA13 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.
  • ab122627 at 4 µg/ml staining SPATA13 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

Anti-SPATA13 antibody (ab122627)参考文献

ab122627 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122627.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"