概述
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产品名称Anti-SPATA13抗体
参阅全部 SPATA13 一抗 -
描述兔多克隆抗体to SPATA13
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宿主Rabbit
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经测试应用适用于: IHC-P, ICC/IFmore details
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种属反应性与反应: Human
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免疫原
Recombinant fragment: ALRPAEWGTL DGSDLEDTDD AFQRSTHRSR SLRRAYGLGR ICLLDAPQNH ATPTIATGQV PAVCEILVRD PENNSMGYRR SKSTDNLAFL KKSSFKRKST SNLADLRTAH of Human SPATA13
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阳性对照
- Human duodenum tissue; U 251 MG cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
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存储溶液pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab122627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization. |
靶标
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功能Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.
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组织特异性Expressed at high levels in the placenta, spleen and kidney, at moderate levels in lung, small intestine, liver, brain and heart, and at low levels in skeletal muscle. Expression is aberrantly enhanced in most colorectal tumors.
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序列相似性Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain. -
结构域The C-terminal tail is required for its GEF activity.
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细胞定位Cytoplasm. Cell projection > filopodium. Cell projection > lamellipodium. Cell projection > ruffle membrane. Accumulates in the lamellipodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
- Information by UniProt
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数据库链接
- Entrez Gene: 221178 Human
- Omim: 613324 Human
- SwissProt: Q96N96 Human
- Unigene: 595391 Human
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别名
- APC-stimulated guanine nucleotide exchange factor 2 antibody
- ARHGEF29 antibody
- Asef2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPATA13 antibody (ab122627)ab122627 at 1/200 dilution staining SPATA13 in paraffin-embedded Human duodenum tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-SPATA13 antibody (ab122627)ab122627 at 4 µg/ml staining SPATA13 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).
文献
ab122627 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"