概述

  • 产品名称Anti-SPARCL1抗体
    参阅全部 SPARCL1 一抗
  • 描述
    兔多克隆抗体to SPARCL1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, between amino acids 215-244 from the Central region of Human SPARCL1 (NP_001121782.1, NP_004675.3).

  • 阳性对照
    • NCI-H460 cell line lysate, Human brain tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab107533 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab107533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 75 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 组织特异性Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes.
  • 序列相似性Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • 翻译后修饰N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Thr-398 is the preferred site to Thr-399.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Ecm2 antibody
    • Extracellular matrix protein 2 antibody
    • Hevin antibody
    • High endothelial venule protein antibody
    • MAST 9 antibody
    • mast9 antibody
    • Matrix glycoprotein Sc1 antibody
    • PIG33 antibody
    • proliferation inducing protein 33 antibody
    • RAGS1 antigen antibody
    • SC1 antibody
    • SPARC like 1 antibody
    • SPARC-like protein 1 antibody
    • Sparcl1 antibody
    • SPRL1_HUMAN antibody
    see all

Anti-SPARCL1 antibody 图像

  • Anti-SPARCL1 antibody (ab107533) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

    Predicted band size : 75 kDa
  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. ab107533 at a dilution of 1/50.

Anti-SPARCL1 antibody (ab107533)参考文献

This product has been referenced in:
  • Hurley PJ  et al. Secreted protein, acidic and rich in cysteine-like 1 (SPARCL1) is down regulated in aggressive prostate cancers and is prognostic for poor clinical outcome. Proc Natl Acad Sci U S A 109:14977-82 (2012). IHC-P . Read more (PubMed: 22927397) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.

I am sorry to confirm that we do not expect a cross reactivity of ab107533 with rat.

The immunogen only shows a 52% homology and we only expect a cross reactivity form 85% homology.

I am sorry t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"