The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Direct ELISA: 1/400 - 1/1000.
IHC-Fr: 1/50 - 1/200.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Belongs to the SPARC family. Contains 1 EF-hand domain. Contains 1 follistatin-like domain. Contains 1 Kazal-like domain.
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.