概述

  • 产品名称
  • 描述
    兔多克隆抗体to SPARC
  • 经测试应用
    适用于: IHC, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from the human SPARC protein.

  • 阳性对照
    • Mouse heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab55847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC Use a concentration of 5 - 10 µg/ml.
ELISA Use a concentration of 1 - 5 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). PubMed: 19509023

靶标

  • 功能
    Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
  • 序列相似性
    Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • 发展阶段
    Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AA517111 antibody
    • Basement membrane protein 40 antibody
    • Basement-membrane protein 40 antibody
    • BM 40 antibody
    • BM-40 antibody
    • BM40 antibody
    • Cysteine rich protein antibody
    • hm:zeh0062 antibody
    • MGC128090 antibody
    • OI17 antibody
    • ON antibody
    • Osteonectin antibody
    • Secreted acidic cystein rich glycoprotein antibody
    • Secreted protein acidic and cysteine rich antibody
    • Secreted protein acidic and rich in cysteine antibody
    • Secreted protein acidic cysteine rich (osteonectin) antibody
    • Secreted protein acidic cysteine rich antibody
    • SPARC antibody
    • SPRC antibody
    • SPRC_HUMAN antibody
    see all

文献

This product has been referenced in:
  • Farrokhi E  et al. Effect of Oxidized Low Density Lipoprotein on the Expression of Runx2 and SPARC Genes in Vascular Smooth Muscle Cells. Iran Biomed J 19:160-4 (2015). Read more (PubMed: 26025968) »
  • Liu Y  et al. Apratoxin a reversibly inhibits the secretory pathway by preventing cotranslational translocation. Mol Pharmacol 76:91-104 (2009). Read more (PubMed: 19403701) »

See all 3 Publications for this product

客户评价及客户问答

Thank you for your enquiry. The 75kDa band could be due to insufficient boiling of the sample, which resulted in the 75kDa dimer band. Since this image is confusing, I have removed it from the datasheet. Please rest assured that the product does de...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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