概述

  • 产品名称
  • 描述
    兔多克隆抗体to SPARC
  • 特异性
    There were no cross reactivities obtained with human Osteopontin, and human Bone Sialoprotein.
  • 经测试应用
    适用于: ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human).

性能

应用

Our Abpromise guarantee covers the use of ab14174 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
  • 序列相似性
    Belongs to the SPARC family.
    Contains 1 EF-hand domain.
    Contains 1 follistatin-like domain.
    Contains 1 Kazal-like domain.
  • 发展阶段
    Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AA517111 antibody
    • Basement membrane protein 40 antibody
    • Basement-membrane protein 40 antibody
    • BM 40 antibody
    • BM-40 antibody
    • BM40 antibody
    • Cysteine rich protein antibody
    • hm:zeh0062 antibody
    • MGC128090 antibody
    • OI17 antibody
    • ON antibody
    • Osteonectin antibody
    • Secreted acidic cystein rich glycoprotein antibody
    • Secreted protein acidic and rich in cysteine antibody
    • Secreted protein acidic cysteine rich (osteonectin) antibody
    • Secreted protein acidic cysteine rich antibody
    • SPARC antibody
    • SPRC antibody
    • SPRC_HUMAN antibody
    see all

Anti-SPARC antibody 图像

  • ab14174 at a 1/100 dilution staining SPARC in mouse endochondral bone by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized with 0.1% Triton. Blocked using 20% serum for 50 minutes at 19°C. Secondary used at 1/200 polyclonal Donkey anti-rabbit conjugated to Alexa Fluor 555 (red). Counterstain (blue) DAPI.

    See Abreview

  • ab14174 (1/1000) staining SPARC in human substanta nigra using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and extracellular matrix.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunohistochemical analysis of E14 and E16 mouse choroid plexus tissue, staining SPARC with ab14174. Sections were blocked with blocking agents and incubated with primary antibody (1/10000) overnight at 4°C. Staining was detected using DAB.

Anti-SPARC antibody (ab14174)参考文献

This product has been referenced in:
  • Waisberg J  et al. Overexpression of the ITGAV gene is associated with progression and spread of colorectal cancer. Anticancer Res 34:5599-607 (2014). Human . Read more (PubMed: 25275062) »
  • Cheng P  et al. Fibronectin mediates mesendodermal cell fate decisions. Development 140:2587-96 (2013). IHC-Fr . Read more (PubMed: 23715551) »

See all 5 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Zebrafish Tissue sections (Skeleton)
Specification
Skeleton
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 12 2014

DISCOUNT CODE: ***
Expiration date: May 30th, 2012

I am very pleased to hear you would like to accept our offer and test ab14174 in pig. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, pl...

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Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Endochondral bone)
Specification
Endochondral bone
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
Serum as blocking agent for 50 minute(s) · Concentration: 20% · Temperature: 19°C
Username

Abcam user community

Verified customer

提交于 Feb 17 2010

Thank you for your enquiry. The immunogen for this antibody is recombinant full length Human protein. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. Ab19528 has been removed from our catalog. Ab14174 is a polyclonal antibody that was raised to the full length SPARC protein. Therefore, the antibody should recognize epitopes along the entire protein. I hope this ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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