The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E.
, corresponding to internal sequence amino acids 31-47 of Human SPANX C
- Nuclear extract from VWM105 cells
Dilute only prior to immediate use.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration information loading...
Immunogen affinity purified
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/10000 - 1/50000.
IF (microscopy): Use at an assay dependent dilution.
WB: 1/500 - 1/3000. Predicted molecular weight: 11 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Detected in testis, melanoma and bladder carcinoma.
Belongs to the SPAN-X family.
Detected in round and elongating spermatids.
Cytoplasm. Nucleus. Associated with nuclear craters.
Information by UniProt
- Cancer-testis-associated protein CTp11 antibody
- Cancer/testis antigen 11.3 antibody
- Cancer/testis-associated protein CTp11 antibody
Western blot - Anti-SPANX antibody (ab47252)
All lanes :
Anti-SPANX antibody (ab47252) at 1/1000 dilutionLane 1 :
Nuclear extract from VWM105 cellsLane 2 :
SPANX-C fusion protein.Lane 3 :
recombinant SPANX-B, protein.Lane 4 :
recombinant SPANX-C, protein.Lane 5 :
recombinant SPANX-N, protein.SecondaryLanes 1-2 :
IRDye™800 conjugated Gt-a-
Rabbit IgG [H&L] MX (611-132-122)Predicted band size:
11 kDaObserved band size:
17 kDa (why is the actual band size different from the predicted?
)Additional bands at:
60 kDa, 70 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"