antigen sequence: MHCGDDEDCF TGHGVAPGTG PVINKGCLRA TSCGLEEPVS YRGVTYSLTT NCCTGRLCNR APSSQTVGAT TSLALGLGML LPPR, corresponding to amino acids 39-122 of Human SPACA4.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization.
Testis specific. Expressed in spermatozoa.
Contains 1 UPAR/Ly6 domain.
Cell membrane. Cytoplasmic vesicle > secretory vesicle > acrosome. Expressed in acrosomal matrix and outer and inner acrosomal membranes.
Information by UniProt
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPACA4 antibody (ab122758)
ab122758, at 1/200 dilution, staining SPACA4 in paraffin-embedded Human testis tissue by Immunohistochemistry.
Western blot - Anti-SPACA4 antibody (ab122758)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
has not yet been referenced specifically in any publications.
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