The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
功能Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.
组织特异性The processed form is expressed in sperm (at protein level). Expressed in testis, epididymis and placenta.
序列相似性Belongs to the glycosyl hydrolase 22 family.
翻译后修饰The processed form derives from the membrane form by proteolytic processing.
细胞定位Secreted and Cytoplasmic vesicle > secretory vesicle > acrosome membrane. Anterior acrosome in non-capacitated spermatozoa and retained in the equatorial segment and in the luminal face of both the inner and outer acrosomal membranes following capacitation and the acrosome reaction.
ab121768 at a 1/2500 dilution staining SPACA3 in paraffin embedded Human testis tissue by Immunohistochemistry.
Western blot - Anti-SPACA3 antibody (ab121768)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells. Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Anti-SPACA3 antibody (ab121768)参考文献
has not yet been referenced specifically in any publications.