Synthetic peptide between amino acids 49-78 from the N terminal region of Human SPACA1 (NP_112222.1), conjugated to KLH.
293 cell line lysate; Human testis tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
ab107976 is purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 32 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May be involved in sperm-egg fusion.
Testis specific. At the equatorial segment and the inner acrosomal membrane of capacitated sperm.
Information by UniProt
Western blot - SPACA1 antibody (ab107976)
Anti-SPACA1 antibody (ab107976) at 1/100 dilution + 293 cell line lysate at 35 µg
Predicted band size: 32 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SPACA1 antibody (ab107976)
ab107976, at a dilution of 1/50, staining SPACA1 in formalin fixed, paraffin embedded Human testis tissue by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"