概述

  • 产品名称Anti-Sp8抗体
    参阅全部 Sp8 一抗
  • 描述
    兔多克隆抗体to Sp8
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Sp8.

    (Peptide available as ab90836.)

  • 阳性对照
    • This antibody gave a positive signal in 46C whole cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab73494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).

靶标

  • 功能Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos.
  • 序列相似性Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BTD antibody
    • Buttonhead antibody
    • Homolog of Btd antibody
    • OTTHUMP00000120017 antibody
    • Sp 8 antibody
    • SP8 antibody
    • Sp8 transcription factor antibody
    • SP8_HUMAN antibody
    • Specificity protein 8 antibody
    • Trans acting transcription factor 8 antibody
    • Transcription factor Sp8 antibody
    see all

Anti-Sp8 antibody 图像

  • Anti-Sp8 antibody (ab73494) at 1 µg/ml + 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 23 kDa,56 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

Anti-Sp8 antibody (ab73494)参考文献

ab73494 has not yet been referenced specifically in any publications.

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