Anti-Sp7 / Osterix抗体- ChIP Grade (ab22552)

概述

  • 产品名称
    Anti-Sp7 / Osterix抗体- ChIP Grade
    参阅全部 Sp7 / Osterix 一抗
  • 描述
    兔多克隆抗体to Sp7 / Osterix - ChIP Grade
  • 经测试应用
    适用于: IHC-Fr, ChIP, ICC/IF, WB, IHC-P, IP, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Mouse Sp7/ Osterix.

    (Peptide available as ab24390.)

  • 阳性对照
    • This antibody gave a positive signal for Immunohistochemistry (FFPE) in Rat bone.

性能

应用

Our Abpromise guarantee covers the use of ab22552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/100.
ChIP Use at an assay dependent concentration. PubMed: 20682789
ICC/IF Use at an assay dependent concentration. PubMed: 20682789
WB Use at an assay dependent concentration. Predicted molecular weight: 46 kDa. PubMed: 17510056
IHC-P 1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.
IP Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20410296

靶标

Anti-Sp7 / Osterix antibody - ChIP Grade 图像

  • ab22552 staining Sp7/ Osterix in C2C12 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were stained with ab22552 at a 1/500 dilution followed by goat anti-rabbit IgG conjugated with Alexa Fluor 488 at a 1/400 dilution. Labeling was detected using a Leica TCS SL inverted laser scanning confocal microscope.

    The subcellular localization of Osterx in C2C12 cells was analyzed by immunofluorescence with ab22552 in cells either transiently transfected with short Osterix construct or incubated in medium without serum. Scale bar 10 µm.
  • Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.

  • ab22552 staining Osterix in Human bone marrow tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with Serum Free Protein Block (Dako) for 5 minutes at 24°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 30 minutes at 24°C. An undiluted HRP-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

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  • Image courtesy of Human Protein Atlas

    ab22552 staining Sp7 / osterix in human bone marrow tissue. Paraffin embedded human bone marrow tissue was incubated with ab22552 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab22552 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .

  • The image shows Osterix staining in rat bone tibiae using ab22552 at 1/100.

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  • ab22552 staining Sp7 / Osterix in 21 days old rat developing long bone tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with methanol and blocking with 20% serum at 220C for 30 minutes was performed. The sample was incubated with primary antibody (1/100) for 3 hours at 220C. A Cy3®-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/1000 dilution.

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Anti-Sp7 / Osterix antibody - ChIP Grade (ab22552)参考文献

This product has been referenced in:
  • Chen Y  et al. An Innovative Approach for Enhancing Bone Defect Healing Using PLGA Scaffolds Seeded with Extracorporeal-shock-wave-treated Bone Marrow Mesenchymal Stem Cells (BMSCs). Sci Rep 7:44130 (2017). WB, IHC-P ; Rat . Read more (PubMed: 28272494) »
  • Pang J  et al. ACVR1-Fc suppresses BMP signaling and chondro-osseous differentiation in an in vitro model of Fibrodysplasia ossificans progressiva. Bone 92:29-36 (2016). WB ; Human . Read more (PubMed: 27492611) »

See all 56 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (calvaria bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Unmasking Solution, Tris-Based Catalog Number: H-3301
Permeabilization
Yes - Triton100
Specification
calvaria bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Tina Yuan

Verified customer

提交于 Dec 19 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6, Citrate based
Permeabilization
No
Specification
Bone
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

提交于 Apr 05 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Bone Marrow)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6, Citrate based
Permeabilization
No
Specification
Bone Marrow
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

提交于 Apr 05 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (periodontal ligament)
Specification
periodontal ligament
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 17 2015

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (10% gel)
Sample
Rat Cell lysate - whole cell (Primary cultured osteoblasts)
Specification
Primary cultured osteoblasts
Blocking step
Membrane blocking agent (GE Healthcare) as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

幸弘 小原

Verified customer

提交于 Jul 24 2014

Application
ChIP on chip
Sample
Mouse Cell lysate - whole cell (mc3t3-e1)
Specification
mc3t3-e1
Type
Native ChIP (N-ChIP)
Detection step
Microarray
Positive control
Osterix transfection in mc3t3-e1 cell line
Negative control
not treated
Username

Abcam user community

Verified customer

提交于 Dec 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (mouse cell)
Total protein in input
500 µg
Specification
mouse cell
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

提交于 Dec 18 2012

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