Anti-Sp7 / Osterix抗体(ab94744)

概述

  • 产品名称Anti-Sp7 / Osterix抗体
    参阅全部 Sp7 / Osterix 一抗
  • 描述
    兔多克隆抗体to Sp7 / Osterix
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (ASSLLEEEVH YGSSPLAMLT AACSKFGGSS PLRDSTTLGK AGTKKPYSVG) of Human Sp7/ Osterix (NP_690599).

  • 阳性对照
    • Fetal lung lysate

性能

应用

Our Abpromise guarantee covers the use of ab94744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-Sp7 / Osterix antibody 图像

  • Anti-Sp7 / Osterix antibody (ab94744) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 45 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling Sp7 / Osterix with ab94744 at 5µg/ml.

Anti-Sp7 / Osterix antibody (ab94744)参考文献

ab94744 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HUVECs)
Gel Running Conditions Reduced Denaturing (9% SDS-PAGE)
Loading amount 20 µg
Treatment 10ng/ml IL-1 beta and 20uM Inhibitor for 15min
Specification HUVECs
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 May 11 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells)
Total protein in input 10 µg
Treatment transfected flag-Osterix construct
Immuno-precipitation step Protein A/G
Specification HeLa cells
Username

Natalia Artigas Robles

Verified customer

提交于 Apr 29 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue (femur)
Specification femur
Username

Abcam user community

Verified customer

提交于 Jan 06 2016

Thank you for contacting us.

Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products. However, I have passes on your details to our distributor (allied scientific). I am currently in contact ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"