概述

性能

应用

Our Abpromise guarantee covers the use of ab57335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

靶标

  • 功能Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.
  • 组织特异性Restricted to bone-derived cell.
  • 序列相似性Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC126598 antibody
    • Osterix antibody
    • Osx antibody
    • Sp 7 antibody
    • SP7 antibody
    • Sp7 transcription factor antibody
    • SP7_HUMAN antibody
    • Transcription factor Sp7 antibody
    • Zinc finger protein osterix antibody
    see all

Anti-Sp7 / Osterix antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab57335 Sp7 / Osterix antibody at 1ug/ml. Predicted band size of immunogen is 34 kDa

Anti-Sp7 / Osterix antibody (ab57335)参考文献

This product has been referenced in:
  • Tang N  et al. Up-regulated osteogenic transcription factors during early response of human periodontal ligament stem cells to cyclic tensile strain. Arch Med Sci 8:422-30 (2012). WB ; Human . Read more (PubMed: 22851995) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (osteoblast cells)
Loading amount 20 µg
Specification osteoblast cells
Treatment BMP-2 for 24hrs
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 May 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"