概述

  • 产品名称Anti-SP3抗体
    参阅全部 SP3 一抗
  • 描述
    兔多克隆抗体to SP3
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide from within residues: AGINADGHLI NTGQAMDSSD NSERTGERVS PDINETNTDT DLFVPTSSSS, corresponding to internal sequence amino acids 288-337 of Human SP3 (NP_003102).

  • 阳性对照
    • Human fetal muscle lysate

性能

应用

Our Abpromise guarantee covers the use of ab87091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone-induction and house-keeping.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 翻译后修饰Not glycosylated.
    Acetylated by histone acetyltransferase p300, deacetylated by HDACs. Acetylation/deacetylation states regulate transcriptional activity. Acetylation appears to activate transcription. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity. Ceramides can also regulate acetylation/deacetylation events through altering the interaction of HDAC with SP3. In vitro, C(18)-ceramides, but not C(16)-ceramides, increase the interaction of HDAC1 with SP3 and enhance the deacetylation of SP3 and the subsequent repression of the TERT promoter.
    Sumoylated on all isoforms. Sumoylated on 2 sites in longer isoforms with Lys-551 being the major site. Sumoylation at this site promotes nuclear localization to the nuclear periphery, nuclear dots and PML nuclear bodies. Sumoylation on Lys-551 represses the transactivation activity, except for the largest isoform, L-Sp3, which has little effect on transactivation. Alternate sumoylation and acetylation at Lys-551 also control transcriptional activity.
  • 细胞定位Nucleus. Nucleus > PML body. Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies.
  • Information by UniProt
  • 数据库链接
  • 别名
    • D130027J01Rik antibody
    • DKFZp686O1631 antibody
    • GC binding transcription factor Sp 3 antibody
    • GC binding transcription factor Sp3 antibody
    • MGC105187 antibody
    • OTTMUSP00000014207 antibody
    • SP 3 antibody
    • Sp 3 transcription factor antibody
    • SP3 antibody
    • Sp3 transcription factor antibody
    • SP3_HUMAN antibody
    • Specificity protein 3 antibody
    • SPR 2 antibody
    • SPR-2 antibody
    • SPR2 antibody
    • Transcription factor SP 3 antibody
    • Transcription factor Sp3 antibody
    see all

Anti-SP3 antibody 图像

  • Anti-SP3 antibody (ab87091) at 1 µg/ml + Fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 82 kDa
    Observed band size : >90 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling SP3 with ab87091 at 5.0µg/ml.

Anti-SP3 antibody (ab87091)参考文献

ab87091 has not yet been referenced specifically in any publications.

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