Anti-SP1 (phospho T453)抗体(ab37707)

概述

  • 产品名称Anti-SP1 (phospho T453)抗体
    参阅全部 SP1 一抗
  • 描述
    兔多克隆抗体to SP1 (phospho T453)
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 450 - 550 of Human SP1, phosphorylated at T453.

    (Peptide available as ab38350.)

  • 阳性对照
    • This antibody gave a positive signal in the following Human whole cell lysates: HeLa and Jurkat.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab37707 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 81 kDa).

靶标

  • 功能Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.
  • 组织特异性Up-regulated in adenocarcinomas of the stomach (at protein level).
  • 序列相似性Belongs to the Sp1 C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • 翻译后修饰Phosphorylated on multiple serine and threonine residues. Phosphorylation is coupled to ubiquitination, sumoylation and proteolytic processing. Phosphorylation on Ser-59 enhances proteolytic cleavage. Phosphorylation on Ser-7 enhances ubiquitination and protein degradation. Hyperphosphorylation on Ser-101 in response to DNA damage has no effect on transcriptional activity. MAPK1/MAPK3-mediated phosphorylation on Thr-453 and Thr-739 enhances VEGF transcription but, represses FGF2-triggered PDGFR-alpha transcription. Also implicated in the repression of RECK by ERBB2. Hyperphosphorylated on Thr-278 and Thr-739 during mitosis by MAPK8 shielding SP1 from degradation by the ubiquitin-dependent pathway. Phosphorylated in the zinc-finger domain by calmodulin-activated PKCzeta. Phosphorylation on Ser-641 by PKCzeta is critical for TSA-activated LHR gene expression through release of its repressor, p107. Phosphorylation on Thr-668, Ser-670 and Thr-681 is stimulated by angiotensin II via the AT1 receptor inducing increased binding to the PDGF-D promoter. This phosphorylation is increased in injured artey wall. Ser-59 and Thr-681 can both be dephosphorylated by PP2A during cell-cycle interphase. Dephosphorylation on Ser-59 leads to increased chromatin association during interphase and increases the transcriptional activity. On insulin stimulation, sequentially glycosylated and phosphorylated on several C-terminal serine and threonine residues.
    Acetylated. Acetylation/deacetylation events affect transcriptional activity. Deacetylation leads to an increase in the expression the 12(s)-lipooxygenase gene though recruitment of p300 to the promoter.
    Ubiquitinated. Ubiquitination occurs on the C-terminal proteolytically-cleaved peptide and is triggered by phosphorylation.
    Sumoylated by SUMO1. Sumoylation modulates proteolytic cleavage of the N-terminal repressor domain. Sumoylation levels are attenuated during tumorigenesis. Phosphorylation mediates SP1 desumoylation.
    Proteolytic cleavage in the N-terminal repressor domain is prevented by sumoylation. The C-terminal cleaved product is susceptible to degradation.
    O-glycosylated; contains at least 8 N-acetylglucosamine side chains. Levels are controlled by insulin and the SP1 phosphorylation states. Insulin-mediated O-glycosylation locates SP1 to the nucleus, where it is sequentially deglycosylated and phosphorylated. O-glycosylation affects transcriptional activity through disrupting the interaction with a number of transcription factors including ELF1 and NFYA. Also inhibits interaction with the HIV1 promoter. Inhibited by peroxisomome proliferator receptor gamma (PPARgamma).
  • 细胞定位Nucleus. Cytoplasm. Nuclear location is governed by glycosylated/phosphorylated states. Insulin promotes nuclear location, while glucagon favors cytoplasmic location.
  • Information by UniProt
  • 数据库链接
  • 别名
    • SP 1 antibody
    • SP1 antibody
    • Sp1 transcription factor antibody
    • SP1_HUMAN antibody
    • Specificity protein 1 antibody
    • Transcription factor Sp1 antibody
    • TSFP 1 antibody
    • TSFP1 antibody
    see all

Anti-SP1 (phospho T453) antibody 图像

  • All lanes : Anti-SP1 (phospho T453) antibody (ab37707) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SP1 (phospho T453) peptide (ab38350) at 1 µg/ml
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SP1 (phospho T453) peptide (ab38350) at 1 µg/ml
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SP1 peptide (ab42654) at 1 µg/ml
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SP1 peptide (ab42654) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 81 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >250 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes
  • ab37707, at a 1/2000 dilution, staining human SP1 (phospho T453) in colon cancer tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.

    See Abreview

  • All lanes : Anti-SP1 (phospho T453) antibody (ab37707) at 1/500 dilution

    Lane 1 : Nuclear lysate prepared from rat intestinal epithelial cells
    Lane 2 : Nuclear lysate prepared from Caco2 cells

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat anti-rabbit polyclonal conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 81 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of Liwei Xie by Abreview.

    See Abreview

Anti-SP1 (phospho T453) antibody (ab37707)参考文献

ab37707 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

We are not sure about the identity of 250kDa band- it's interesting that some other vendors abs also show same additional band.

I have blasted the immunogen sequence against Human, the immunogen is specifi...

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Thank you for contacting us.

The link to the protocol for blocking with the immunogen peptide can be found here:

http://www.abcam.com/index.html?pageconfig=resource&rid=11378

For your convenience, I have also attache...

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Thank you for contacting us.
I am sorry that the antibody is giving additional bands.

As we discussed over the phone, the following suggestions might be tried:

- use reducing conditions
- block overnight
-use less pri...

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Thank you for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab59257 on the order ***, which should arrive on Monday.

Please keep me updated about ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - nuclear (intestine)
Loading amount 50 µg
Specification intestine
Gel Running Conditions Non-reduced Denaturing (SDS)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Liwei Xie

Verified customer

提交于 Jul 14 2011

SP1 has a number of glycosylation sites and so this is probably the reason why we see a difference between the expected MW (80-90kDa) (ab59257) and the observed (120kDa) in our product ab37707. We have run ab37707 alongside ab59257. No information wa...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon cancer)
Specification Colon cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

提交于 May 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"