The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
SRY (sex-determining region Y)-box 9 protein antibody
SRY related HMG box gene 9 antibody
Transcription factor SOX 9 antibody
Transcription factor SOX-9 antibody
transcription factor SOX9 antibody
Western blot - SOX9 (phospho S181) antibody (ab68610)
All lanes : Anti-SOX9 (phospho S181) antibody (ab68610) at 1 µg/ml
Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue Lane 2 : Human liver tissue lysate - total protein (ab29889) Lane 3 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
with Modified blocking peptide at 1 µg/ml Lane 4 : Human liver tissue lysate - total protein (ab29889) with Modified blocking peptide at 1 µg/ml Lane 5 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
with Non - Modified blocking peptide at 1 µg/ml Lane 6 : Human liver tissue lysate - total protein (ab29889) with Non - Modified blocking peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 56 kDa Observed band size : 56 kDa Additional bands at : 15 kDa,40 kDa. We are unsure as to the identity of these extra bands.