Anti-SOX9 (phospho S181)抗体(ab59252)

概述

  • 产品名称Anti-SOX9 (phospho S181)抗体
    参阅全部 SOX9 一抗
  • 描述
    兔多克隆抗体to SOX9 (phospho S181)
  • 特异性Detects endogenous levels of SOX9 only when phosphorylated at serine 181.
  • 经测试应用适用于: ELISA, IHC-P, WB, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human
  • 免疫原

    Synthetic phosphopeptide derived from human SOX9 around the phosphorylation site of serine 181 (R-K-SP-V-K).

  • 阳性对照
    • Human brain.

性能

应用

Our Abpromise guarantee covers the use of ab59252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 23382206
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • 疾病相关Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • 序列相似性Contains 1 HMG box DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Anti-SOX9 (phospho S181) antibody 图像

  • All lanes : Anti-SOX9 (phospho S181) antibody (ab59252) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with PBS (60mins)
    Lane 2 : 293 cell extracts treated with PBS (60mins) with immunizing phospho-peptide


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab59252 at 1/50 dilution staining SOX9 in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Anti-SOX9 (phospho S181) antibody (ab59252)参考文献

This product has been referenced in:
  • Jacob W  et al. Decreased adipogenesis and adipose tissue in mice with inactivated protein phosphatase 5. Biochem J 466:163-76 (2015). Read more (PubMed: 25437352) »
  • Guha Thakurta S  et al. Growth factor and ultrasound-assisted bioreactor synergism for human mesenchymal stem cell chondrogenesis. J Tissue Eng 6:2041731414566529 (2015). WB ; Human . Read more (PubMed: 25610590) »

See all 7 Publications for this product

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Indeed we can provide discount codes for the value of 100% discount off the next purchase to customers submitting Abreviews for testing our antibodies in new species / applica...

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Application Western blot
Sample Cow Cell lysate - whole cell (chondrocytes lysate in RIPA buffer)
Loading amount 20 µg
Specification chondrocytes lysate in RIPA buffer
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 37°C
Username

Dr. Nazish Ahmed

Verified customer

提交于 Dec 02 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human cells)
Loading amount 30 µg
Specification human cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Michaela Leyh

Verified customer

提交于 Feb 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"