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Our Abpromise guarantee covers the use of ab3697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/200. PubMed: 19388005|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 56 kDa).|
|IP||Use 3-5µg for 107 cells.|
|ELISA||Use a concentration of 0.1 - 1 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent concentration. PubMed: 21872590|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin hair follicles labling SOX9 with ab3697. Hematoxylin purple nuclear conterstain.
Rabbit polyclonal antibody to Sox9 recognizes full length of Sox9 (left lane) and C-terminal deletion of Sox9 (right lane)
(Compared to anti-Sox9 antibody from a different company - not shown).
This Western blot image was kindly supplied as part of the review submitted by Lilia Topol.