Anti-SOX9抗体- ChIP Grade (ab3697)

概述

  • 产品名称Anti-SOX9抗体- ChIP Grade
    参阅全部 SOX9 一抗
  • 描述
    兔多克隆抗体to SOX9 - ChIP Grade
  • 特异性The antibodies were evaluated for specificity with a dot blot assay using synthetically prepared SOX9 peptides. By immunoblot analysis, a ~65 kDa immunoreactive protein from mouse embryo cell lysate was detected (sample were boiled prior to subject 10% SDS-polyacrylamide gel electrophoresis). This band is abolished by the depletion of the synthetic peptide.
  • 经测试应用适用于: IHC-Fr, ICC/IF, WB, IP, ELISA, IHC-P, ChIPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow
  • 免疫原

    Synthetic peptide:

    KYQPRRRKSVKNG

    , corresponding to amino acids 173 - 185 of Human/ Mouse/ Bovine SOX 9.

  • 阳性对照
    • 3T3 cells FFPE mouse e14 whole foetus tissue sections

应用

Our Abpromise guarantee covers the use of ab3697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/200. PubMed: 19388005
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 56 kDa).
IP Use 3-5µg for 107 cells.
ELISA Use a concentration of 0.1 - 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIP Use at an assay dependent concentration. PubMed: 21872590

靶标

  • 功能Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • 疾病相关Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • 序列相似性Contains 1 HMG box DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Anti-SOX9 antibody - ChIP Grade 图像



  • Predicted band size : 56 kDa

    Rabbit polyclonal antibody to Sox9 recognizes full length of Sox9 (left lane) and C-terminal deletion of Sox9 (right lane)

    (Compared to anti-Sox9 antibody from a different company - not shown).

    This Western blot image was kindly supplied as part of the review submitted by Lilia Topol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin hair follicles labling SOX9 with ab3697. Hematoxylin purple nuclear conterstain.

  • ChIP analysis of 15P-1 cells using no antibody (lane 1), a control rabbit IgG (lane 2) or ab3697 (lane 3). DNA from the Er71 promoter (-334 to +28), an Er71 region ranging from exon 6 to exon 7, or the 3'-UTR of Sox9 (2664–3023) was PCR amplified.
  • IHC-P image of SOX9 (ab3697) on E11.5 mouse embryo sections. The sections were fixed in paraformaldehyde and underwent heat mediated antigen retrieval using Tris/EGTA (pH9). The sections were then blocked in 1% BSA solution for 30 mins at 20°C. In these images, ab3697 can be seen staining nuclei on the lumen surface of the brain (green).

    See Abreview

  • Immunofluroescence analysis of murine nucleus pulposus (NP) cells, staining SOX9 with ab3697.
    Left panel = 2 day vehicle treated control
    Right panel = 2 day cyclopamine treated cells
  • Immunofluroescence analysis of murine nucleus pulposus (NP) cells, staining SOX9 with ab3697.
    Left panel = control Shh flox mouse
    Right panel = Shh mutant mouse
  • IHC image of SOX9 staining in mouse e14 whole foetus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3697, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SOX9 antibody - ChIP Grade (ab3697)参考文献

This product has been referenced in:
  • Lu W  et al. CXCL12/CXCR4 Axis Regulates Aggrecanase Activation and Cartilage Degradation in a Post-Traumatic Osteoarthritis Rat Model. Int J Mol Sci 17:N/A (2016). Rat . Read more (PubMed: 27690009) »
  • Yao Y  et al. MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia. Stem Cell Reports 7:249-62 (2016). WB ; Human . Read more (PubMed: 27509135) »

See all 19 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human Chondrocyte cell line)
Specification Human Chondrocyte cell line
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 1000µg/mL · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 09 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6.1
Sample Zebrafish Tissue sections (Juvenile zebrafish head including gills)
Specification Juvenile zebrafish head including gills
Permeabilization Yes - 70% Methanol/3% H2O2, room temp x 20 min
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 20 2014

We do not have any EMSA data for this SOX9 antibody, and we are not aware of any references or feedback from customers who have used it for EMSA. There are two references listed on the datasheet that have used it for ChIP. These might help convince you...

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I do think IP is a good idea, though you will see some strong signal at 50 and 25 kDa, if your secondary anti-rabbit secondary IgG detects denatured rabbit IgG, which will be in the IP product. It may not be a problem if the band of interest is near 56...

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Thank you for contacting us.

I have checked our database and the immunogen sequence of ab3697 is indeed xxxxxxxx, corresponding to amino acids xxxxxx of Human / Mouse / Bovine SOX 9.

I have checked the sequence homology between hum...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E11.5 embryo)
Specification E11.5 embryo
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris/EGTA pH=9
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 07 2012

Thank you for contacting us with your question.

Ab26414 works well with formalin-fixed, paraffin-embedded sections but it has not been sucessfully used with cryosections, so I wouldn't recommend using it in IHC-Fr.

Ab3697 has been t...

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I appreciate the time taken to submit further information to us.
Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results.
I apologise for the inconvenience and am pleased to offer yo...

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Thank you for contacting us.
I am sorry to hear you have been experiencing problems with one of our antibodies. The quality of our products is important to us and I would like to reassure you that we investigate all customer complaints. I apprecia...

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Thank you for contacting us. I am sorry to hear your customer is not obtaining the expected results with ab3697. Having reviewed the protocol there are a few things that I can suggest which may help improve the results currently observed. First...

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