Anti-SOX8抗体(ab125858)

概述

  • 产品名称Anti-SOX8抗体
    参阅全部 SOX8 一抗
  • 描述
    兔多克隆抗体to SOX8
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chicken, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human SOX8.

  • 阳性对照
    • This antibody gave a positive signal in both Human Testis and Human Colon tissue lysates.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能May play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'.
  • 序列相似性Contains 1 HMG box DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC24837 antibody
    • SOX 8 antibody
    • Sox8 antibody
    • SOX8_HUMAN antibody
    • SRY (sex determining region Y) box 8 antibody
    • SRY box 8 antibody
    • SRY box containing gene 8 antibody
    • Transcription factor SOX 8 antibody
    • Transcription factor SOX-8 antibody
    • Transcription factor SOX8 antibody
    see all

Anti-SOX8 antibody 图像

  • All lanes : Anti-SOX8 antibody (ab125858) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,255 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • All lanes : Anti-SOX8 antibody (ab125858) at 1/700 dilution

    Lane 1 : Glioblastoma cell line expressing high Sox8 according to RealTime PCR data.
    Lane 2 : Glioblastoma cell line expressing low Sox8 according to RealTime PCR data.
    Lane 3 : Glioblastoma cell line expressing low Sox8 according to RealTime PCR data.

    Secondary
    Standard HRP secondary at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image was taken from an anonymous abreview.

    See Abreview

Anti-SOX8 antibody (ab125858)参考文献

ab125858 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma cell lines)
Loading amount 5 µg
Specification Glioblastoma cell lines
Gel Running Conditions Reduced Denaturing (8% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
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提交于 Mar 21 2012

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