Anti-SOX8抗体(ab125858)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to SOX8
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chicken, Macaque monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human SOX8.

  • 阳性对照
    • This antibody gave a positive signal in both Human Testis and Human Colon tissue lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab125858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).

靶标

  • 功能
    May play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'.
  • 序列相似性
    Contains 1 HMG box DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MGC24837 antibody
    • SOX 8 antibody
    • Sox8 antibody
    • SOX8_HUMAN antibody
    • SRY (sex determining region Y) box 8 antibody
    • SRY box 8 antibody
    • SRY box containing gene 8 antibody
    • Transcription factor SOX 8 antibody
    • Transcription factor SOX-8 antibody
    • Transcription factor SOX8 antibody
    see all

图片

  • All lanes : Anti-SOX8 antibody (ab125858) at 1 µg/ml

    Lane 1 : Human testis tissue lysate - total protein (ab30257)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 105 kDa,255 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • All lanes : Anti-SOX8 antibody (ab125858) at 1/700 dilution

    Lane 1 : Glioblastoma cell line expressing high Sox8 according to RealTime PCR data.
    Lane 2 : Glioblastoma cell line expressing low Sox8 according to RealTime PCR data.
    Lane 3 : Glioblastoma cell line expressing low Sox8 according to RealTime PCR data.

    Secondary
    Standard HRP secondary at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image was taken from an anonymous abreview.

    See Abreview

实验方案

文献

ab125858 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cell lines)
Loading amount
5 µg
Specification
Glioblastoma cell lines
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 4°C
Username

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提交于 Mar 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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