Anti-SOX6抗体- ChIP Grade (ab64946)

概述

  • 产品名称Anti-SOX6抗体- ChIP Grade
    参阅全部 SOX6 一抗
  • 描述
    兔多克隆抗体to SOX6 - ChIP Grade
  • 特异性This antibody detects endogenous levels of total SOX6 protein.
  • 经测试应用适用于: ChIP/Chip, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) from the N terminus of human SOX6.

  • 阳性对照
    • Jurkat cell extract

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab64946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP/Chip Use at an assay dependent dilution.
WB 1/500 - 1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
ELISA 1/5000.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
  • 组织特异性Expressed in a wide variety of tissues, most abundantly in skeletal muscle.
  • 序列相似性Contains 1 HMG box DNA-binding domain.
  • 翻译后修饰Sumoylation inhibits the transcriptional activity.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HSSOX 6 antibody
    • HSSOX6 antibody
    • SOX 6 antibody
    • sox6 antibody
    • SOX6_HUMAN antibody
    • SOXD antibody
    • SRY (sex determining region Y) box 6 antibody
    • SRY box 6 antibody
    • SRY box containing gene 6 antibody
    • Transcription factor SOX 6 antibody
    • Transcription factor Sox-6 antibody
    • Transcription factor SOX6 antibody
    see all

Anti-SOX6 antibody - ChIP Grade 图像

  • All lanes : Anti-SOX6 antibody - ChIP Grade (ab64946) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 93 kDa
    Observed band size : 93 kDa

Anti-SOX6 antibody - ChIP Grade (ab64946)参考文献

This product has been referenced in:
  • Mitchell J  et al. ß2-adrenergic receptors inhibit the expression of collagen type II in growth plate chondrocytes by stimulating the AP-1 factor Jun-B. Am J Physiol Endocrinol Metab 300:E633-9 (2011). WB ; Mouse . Read more (PubMed: 21177286) »
  • Xu J  et al. Transcriptional silencing of {gamma}-globin by BCL11A involves long-range interactions and cooperation with SOX6. Genes Dev 24:783-98 (2010). WB, ChIP/Chip ; Human . Read more (PubMed: 20395365) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry.
Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.
We aim to provide as much information as possible to our customers, so I am sorry that...

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Thank you for your enquiry regarding ab64946 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
I would like to reassure you that our Abpromise applie...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.
As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet...

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Thank you for contacting Abcam.
The antibody should be completely fine with being stored at 4C for a week, you should not see an adverse affects with the performance of the antibody. If the antibody was stored for longer at 4C rather than -20C, th...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lung, A549 cell line)
Specification Lung, A549 cell line
Fixative Paraformaldehyde
Permeabilization Yes - TRITON .25%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Apr 09 2012

Thank you very much for your interest in our SOX6 antibodies.

I am happy to let you know that we have an anti-SOX6 antibody available that is guaranteed to work in ChIP with human, mouse or rat samples:
ab64946 : Click here (or use the ...

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Thank you for confirming the order information. I have processed the customer’s request to receive ab33875 as a replacement antibody for ab64649 which did not work in western blot. If there is anything else I can help you with, please let me know.

Thank you for your reply. I would be happy to send ab33875 as a replacement. I think I have found the original order but I would just like to confirm before placing the replacement request. Was the order number ****** and was it placed on the 29th June...

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Thank you for your reply. I am sorry about the confusion over the block, I misread your notes that you sent, as I thought that you were blocking in 5% Milk and incubating the primary in BSA and I thought that by blocking and incubating in the same buff...

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Thank you for contacting Abcam. I am sorry that you are experiencing trouble with ab64946 in western blot. From the information and images you provided I have a few suggestions that may help reduce background and increase specificity. 1 - I would recom...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"