概述

  • 产品名称
  • 描述
    兔多克隆抗体to SOX6
  • 特异性
    This antibody has been tested in IHC-P in wildtype post-natal mouse brain sections and showed specific staining. No immunoreactivity was observed in control samples of post-natal brain from a Sox6 knockout mouse.
  • 经测试应用
    适用于: IHC-P, IP, WB, IHC - Wholemount, ChIP, IHC-FoFr, CHIPseqmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Mouse SOX6.

    (Peptide available as ab30530.)

性能

应用

Our Abpromise guarantee covers the use of ab30455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/1000. Antigen retrieval is not essential but may optimise staining.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration. PubMed: 21985497
IHC-FoFr 1/300.
CHIPseq Use at an assay dependent concentration. PubMed: 21985497

靶标

  • 功能
    Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
  • 组织特异性
    Expressed in a wide variety of tissues, most abundantly in skeletal muscle.
  • 序列相似性
    Contains 1 HMG box DNA-binding domain.
  • 翻译后修饰
    Sumoylation inhibits the transcriptional activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HSSOX 6 antibody
    • HSSOX6 antibody
    • SOX 6 antibody
    • sox6 antibody
    • SOX6_HUMAN antibody
    • SOXD antibody
    • SRY (sex determining region Y) box 6 antibody
    • SRY box 6 antibody
    • SRY box containing gene 6 antibody
    • Transcription factor SOX 6 antibody
    • Transcription factor Sox-6 antibody
    • Transcription factor SOX6 antibody
    see all

Anti-SOX6 antibody 图像

  • ab30455 staining Sox6 in post-natal mouse brain sections. PFA-fixed post-natal mouse brain sections were incubated for 30 minutes in blocking solution followed by an overnight incubation with ab30455 (1/500) at 4°C. No staining was observed in sections obtained from Sox6 knockout mice.
  • IHC-FoFr image of Sox6 staining in Rat hippocampus sections using ab30455.The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. immunostainings were performed using the ‘free floating’ technique.

    See Abreview

Anti-SOX6 antibody (ab30455)参考文献

This product has been referenced in:
  • Suchorska WM  et al. Comparison of Four Protocols to Generate Chondrocyte-Like Cells from Human Induced Pluripotent Stem Cells (hiPSCs). Stem Cell Rev 13:299-308 (2017). ICC/IF ; Human . Read more (PubMed: 27987073) »
  • Lee WJ  et al. An Integrative Developmental Genomics and Systems Biology Approach to Identify an In Vivo Sox Trio-Mediated Gene Regulatory Network in Murine Embryos. Biomed Res Int 2017:8932583 (2017). CHIPseq ; Mouse . Read more (PubMed: 28630873) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse cortical astrocytes)
Specification
mouse cortical astrocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Radhika Menon

Verified customer

提交于 Sep 06 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
brain
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Feb 03 2016

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Mouse Tissue lysate - whole (Mouse cortex)
Specification
Mouse cortex
Total protein in input
20 µg
Username

Abcam user community

Verified customer

提交于 Dec 31 2014

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Human Tissue lysate - whole (HEK293T cells)
Specification
HEK293T cells
Treatment
Myc-tagged Sox6 co-transfected with E1, E2 or E3 ligases
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Oct 24 2014

Abreviews
Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Tissue lysate - nuclear (Primary chondrocytes)
Specification
Primary chondrocytes
Negative control
DNA primers targeting non-binding region
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
DNA primers targeting promoter region
Username

Abcam user community

Verified customer

提交于 Oct 23 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Mouse Cell lysate - whole cell (Muscle C2C12 cells)
Specification
Muscle C2C12 cells
Treatment
100 μg/ml cycloheximide (CHX)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Oct 23 2014

Application
IHC - Wholemount
Sample
Mouse Tissue (Mouse limb muscle)
Specification
Mouse limb muscle
Username

Abcam user community

Verified customer

提交于 May 02 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 23°C
Sample
Mouse Cell (NIH 3T3 fibroblasts)
Specification
NIH 3T3 fibroblasts
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 02 2014

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Sample
Mouse Tissue sections (Mouse epidermis)
Specification
Mouse epidermis
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 02 2014

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Rat Cell lysate - whole cell (C2C12 cell line)
Specification
C2C12 cell line
Total protein in input
500 µg
Username

Abcam user community

Verified customer

提交于 Apr 07 2014

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