Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 778-828 of Human SOX6 (EAW68458.1).
293T, HeLa and Jurkat whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8.
Concentration information loading...
Immunogen affinity purified
ab125196 was affinity purified using an epitope specific to SOX6 immobilized on a solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 92 kDa.
Use at 2-10 µg/mg of lysate.
Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
Expressed in a wide variety of tissues, most abundantly in skeletal muscle.
Contains 1 HMG box DNA-binding domain.
Sumoylation inhibits the transcriptional activity.
Information by UniProt
HSSOX 6 antibody
SOX 6 antibody
Western blot - Anti-SOX6 antibody (ab125196)
All lanes : Anti-SOX6 antibody (ab125196) at 0.4 µg/ml Lane 1 : 293T whole cell lysate at 50 µg Lane 2 : 293T whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 50 µg Lane 4 : Jurkat whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 92 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-SOX6 antibody (ab125196)
Detection of SOX6 by Western Blot of Immunprecipitate.
ab125196 at 1µg/ml staining SOX6 in HeLa whole cell lysate immunoprecipitated using ab125196 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"