Anti-SOX5抗体- ChIP Grade (ab94396)

概述

  • 产品名称Anti-SOX5抗体- ChIP Grade
    参阅全部 SOX5 一抗
  • 描述
    兔多克隆抗体to SOX5 - ChIP Grade
  • 经测试应用适用于: ChIP, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Chicken, Cow, Pig, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SOX5.

  • 阳性对照
    • This antibody gave a positive signal in Human Brain Tissue Lysate.

性能

应用

Our Abpromise guarantee covers the use of ab94396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use at an assay dependent concentration. PubMed: 22547066
IHC-Fr 1/200.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).

靶标

Anti-SOX5 antibody - ChIP Grade 图像

  • ab94396 staining SOX5 in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab94396 staining SOX5 in mouse cartilage tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 1% Triton in PBS and blocked with 10% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 10% serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • Anti-SOX5 antibody - ChIP Grade (ab94396) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 24 kDa,28 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

Anti-SOX5 antibody - ChIP Grade (ab94396)参考文献

This product has been referenced in:
  • Yamashita S  et al. L-Sox5 and Sox6 Proteins Enhance Chondrogenic miR-140 MicroRNA Expression by Strengthening Dimeric Sox9 Activity. J Biol Chem 287:22206-15 (2012). ChIP ; Mouse . Read more (PubMed: 22547066) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chicken Tissue sections (3 day old chick embryo)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization Yes - 0.5% Triton
Specification 3 day old chick embryo
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Christina Murko

Verified customer

提交于 Aug 14 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer at 95C for 10 min
Sample Mouse Tissue sections (brain)
Specification brain
Permeabilization Yes - triton X-100
Fixative Paraformaldehyde
Username

Karen Yee

Verified customer

提交于 Aug 08 2014

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (Cartilage)
Specification Cartilage
Permeabilization Yes - 1% triton in PBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 20 2014

Vielen Dank für Ihren Anruf.



Leider habe ich eine schlechte Nachricht für Sie: Aggrecan hat insgesamt eine Übereinstimmung von 19%, es gibt eine Region zwischen AS 800-850 des menschlichen Proteins, welches eine hoh...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"