概述

  • 产品名称Anti-SOX5抗体
    参阅全部 SOX5 一抗
  • 描述
    兔多克隆抗体to SOX5
  • 经测试应用适用于: IHC-Fr, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Chicken, Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: PEPGMPVIQS TYGVKGEEPH IKEEIQAEDI NGEIYDEYDE EEDDPDVDYG , corresponding to C terminal amino acids 702-751 of isoform 1, or 316-365 of isoform 3 of Human SOX5(Peptide available as ab104436.)

  • 阳性对照
    • HepG2 cell lysate and human lung

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab26041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/500. PubMed: 19521496
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/62500.

靶标

Anti-SOX5 antibody 图像

  • ab26041 at a concentration of 4-8 µg/ml staining human SOX5 by Immunohistochemistry in paraffin embedded lung tissue. Cells with positive label (as indicated by the arrows) are alveclar cells. Magnification 400X.
  • ab26041 at 1/500 dilution staining SOX5 in chicken embryo head section by immunohistochemistry (Frozen sections). Sections were incubated overnight in 30% sucrose in PBS at 4°C, embedded in OCT freezing medium, frozen and sectioned in a cryostat. After fixation sections were rehydrated in PBS for 15 min and then blocked in PBS containing 1% fetal calf serum, 0.1% Triton-X and 0.02% Thimerosal. Primary antibody was used overnight at 4°C. The secondary antibody was used at 1/200 dilution for 2 hours.

Anti-SOX5 antibody (ab26041)参考文献

This product has been referenced in:
  • Aza-Carmona M  et al. NPPB and ACAN, two novel SHOX2 transcription targets implicated in skeletal development. PLoS One 9:e83104 (2014). WB . Read more (PubMed: 24421874) »
  • Fernandes AM  et al. Similar properties of chondrocytes from osteoarthritis joints and mesenchymal stem cells from healthy donors for tissue engineering of articular cartilage. PLoS One 8:e62994 (2013). IHC-P ; Human . Read more (PubMed: 23671648) »

See all 10 Publications for this product

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Leider habe ich eine schlechte Nachricht für Sie: Aggrecan hat insgesamt eine Übereinstimmung von 19%, es gibt eine Region zwischen AS 800-850 des menschlichen Proteins, welches eine hoh...

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Application Western blot
Sample Human Cell lysate - nuclear (progenitor cells)
Loading amount 100 µg
Specification progenitor cells
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Jun 04 2010

Thank you for your enquiry. This antibody was produced outside of Abcam, and the originator considers the exact immunogen sequence proprietary. Thus I cannot give you the exact sequence. The peptide was derived from a region between residues 286 -...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"