Anti-SOX4抗体(ab80261)

概述

  • 产品名称Anti-SOX4抗体
    参阅全部 SOX4 一抗
  • 描述
    兔多克隆抗体to SOX4
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human SOX4 (N terminal). Immunogen located within the region from aa 38 and 87.
    Database link: Q06945

  • 阳性对照
    • HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab80261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-SOX4 antibody 图像

  • Anti-SOX4 antibody (ab80261) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration 12%.
  • ICC/IF image of ab80261 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80261, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • ab80261 at 5 µg/ml staining SOX4 in Human heart tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab80261 at 5 µg/ml staining SOX4 in Human testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-SOX4 antibody (ab80261)参考文献

ab80261 has not yet been referenced specifically in any publications.

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