Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX21 antibody (ab24604)This image is courtesy of Carl Hobbs, King's College London, United Kingdom
Immunohistochemistry (Formaldehyde/PFA-fixed paraffin-embedded sections) using SOX21 antibody (ab24604)on Rat Tissue sections (adult brain lateral ventricle subventricular zone). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT°C. ab24604 used at 1/300 incubated for 16 hours. Secondary Antibody: biotin conjugated anti rabbit IgG (1/300). NB: Sox21 protein expression showin in this image is in concurrs with published mRNA positivity in the subventricular zone and rostro-migratory stream in the adult rat.