Anti-SOX2抗体- Embryonic Stem Cell Marker (ab75179)

概述

  • 产品名称Anti-SOX2抗体- Embryonic Stem Cell Marker
    参阅全部 SOX2 一抗
  • 描述
    兔多克隆抗体to SOX2 - Embryonic Stem Cell Marker
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 SOX2.

    (Peptide available as ab15831.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates; MEF1, MEL-1, F9, Mouse EG (TMAS), E14Tg2a, Hues7, 46C.
  • 常规说明ab75179 is raised using the same immunogen as ab15830. Batches sold under ab75179 have passed QC testing in Western Blot only. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab75179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).Can be blocked with Human SOX2 peptide (ab15831).

靶标

  • 功能Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • 疾病相关Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • 序列相似性Contains 1 HMG box DNA-binding domain.
  • 翻译后修饰Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Anti-SOX2 antibody - Embryonic Stem Cell Marker 图像

  • All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179) at 1 µg/ml

    Lane 1 : Hela Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 4 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
    Lane 5 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 8 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 190 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179) at 1 µg/ml

    Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : 46C (Mouse neural progenitor, selected for Sox1 expression cell line) Whole Cell Lysate (ab27205)
    Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

Anti-SOX2 antibody - Embryonic Stem Cell Marker (ab75179)参考文献

This product has been referenced in:
  • Cox JL  et al. Banf1 is required to maintain the self-renewal of both mouse and human embryonic stem cells. J Cell Sci 124:2654-65 (2011). WB ; Human, Mouse . Read more (PubMed: 21750191) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application IHC - Wholemount
Sample Leucoraja erinacea Embryo (Whole embryo)
Specification Whole embryo
Username

Dr. Andrew Gillis

Verified customer

提交于 Jul 24 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse embryonic brain sections (E10.5))
Specification mouse embryonic brain sections (E10.5)
Fixative 4% PFA
Permeabilization Yes - 0.05% PBS-T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. veronique marthiens

Verified customer

提交于 Aug 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"