概述

  • 产品名称Anti-SOX2抗体
    参阅全部 SOX2 一抗
  • 描述
    兔多克隆抗体to SOX2
  • 经测试应用适用于: IHC-P, ICC/IF, IP, WB, IHC - Wholemount, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human, Xenopus laevis, Zebrafish, Quail, Rainbow Trout, Spotted Catshark (Scyliorhinus canicula), Thornback ray
    预测可用于: Sheep, Horse, Cow, Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human SOX2.

    (Peptide available as ab97974.)

  • 阳性对照
    • This antibody gave a positive signal in NCCIT, F9 and C6 Whole Cell Lysates.

性能

应用

Our Abpromise guarantee covers the use of ab97959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/2000.
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34,40 kDa (predicted molecular weight: 34 kDa).
IHC - Wholemount 1/200.
IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • 疾病相关Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • 序列相似性Contains 1 HMG box DNA-binding domain.
  • 翻译后修饰Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Anti-SOX2 antibody 图像

  • Cell line: NCCIT (human pluripotent embryonal carcinoma)

    Target AbID: Ab97959 anti-Sox2, Ab150077 AlexaFluor®488 Goat anti-Rabbit secondary

    Counterstain AbID: Ab7291 anti-Tubulin (Rabbit mAb), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary

    Fixative: 4% PFA

    Permeabilisation: 0.1% Triton-X

    Nuclear counter stain: DAPI

    Comments: Confocal image showing negative staining on NCCIT cells

    Target primary antibody dilution: 1:500

    Target secondary antibody dilution: 1:1000 (2ug/mL)

    Counterstain primary antibody dilution: 1:1000 (1ug/mL)

    Counterstain secondary antibody dilution: 1:1000 (2ug/mL)

    Negative control 1 primary antibody dilution: 1:500 (Ab97959)

    Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150120)

    Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab7291)

    Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150077)

  • ICC/IF image of ab97959 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97959, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • IHC-P image of SOX2 (ab97959) on E13 mouse Spinal Cord sections. The sections were fixed in formaldehyde and underwent heat mediated Anigen retrieval using citric acis (pH6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C.

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  • All lanes : Anti-SOX2 antibody (ab97959) at 1 µg

    Lane 1 : NCCIT (Human embryonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : C6 (Rat glioma cell line) Whole Cell Lysate
    Lane 5 : Hippocampus (Rat) Tissue Lysate
    Lane 6 : Spinal Cord (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Blots were developed with goat anti-rabbit IgG (H+L) and goat anti-mouse IgG (H+L) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    Green signal from target - ab97959 observed at 40 kDa
    Red signal from loading control ab9484 (GAPDH) observed at 37 kDa

  • IHC-P image of SOX2 (ab97959) on zebrafish developing retina sections. The sections were fixed in formaldehyde and underwent heat mediated Anigen retrieval using citric acis (pH6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C. The upper section used Abcam's EXPOSE HRP-DAB polyvalent kit (ab80436). The lower section was immunostained using my std stABCpx-DAB method.

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  • All lanes : Anti-SOX2 antibody (ab97959) at 1 µg/ml

    Lane 1 : IOUD2 (Mouse embryonic stem cell) Whole Cell Lysate
    Lane 2 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,39 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • IHC-P image of SOX2 (ab97959) on E7 Chicken brain sections. The sections were fixed in formaldehyde and underwent heat mediated Anigen retrieval using citric acis (pH6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C. The upper section used Abcam's EXPOSE HRP-DAB polyvalent kit (ab80436). The lower section was immunostained using my std stABCpx-DAB method.

    See Abreview

  • IHC-P image of SOX2 (ab97959) on Adult rat brain sections. The sections were fixed in formaldehyde and underwent heat mediated Anigen retrieval using citric acis (pH6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C. Upper image using EXPOSE HRP-DAB polyvalent kit (ab80436) shows strong, clear immunostaining of many nuclei in the subventricular region of the lateral ventricle ( double-red arrowhead) and also of the ependymal cells lining the ventricle ( black arrowheads). Direction of migration indicated by green arrows.

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  • IHC-P image of SOX2 (ab97959) on E9 Quail developing retina sections. The sections were fixed in formaldehyde and underwent heat mediated Anigen retrieval using citric acis (pH6). The sections were then blocked in 1% BSA solution for 10 mins at 21°C.

    See Abreview

  • ab97959 staining SOX2 in Spotted Catshark (Scyliorhinus canicula) eye tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/500 in TBS + 1% BSA) for 12 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

    See Abreview

  • IHC-Wholemount image of anti-Sox2 antibody (ab97959) staining on a mouse embryo. This embryo was stained with anti-Sox2 antibody (green) for 48 hours at 4°C. Nuclei stained with DAPI (grey).

    See Abreview

  • IHC - Wholemount of Xenopus laevis embryo labelling SOX2 with ab697959. Sample was incubated with primary antibody (1/250) for 12 hours at 4°C. An Alkaline Phosphatase-conjugated mouse anti-rabbit IgG monoclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • Cell line: NIH/3T3 (mouse embryonic fibroblast cell line)

    Target AbID: Ab97959 anti-Sox2, Ab150077 AlexaFluor®488 Goat anti-Rabbit secondary

    Counterstain AbID: Ab7291 anti-Tubulin (Rabbit mAb), Ab150120 AlexaFluor®594 Goat anti-Mouse secondary

    Fixative: 4% PFA

    Permeabilisation: 0.1% Triton-X

    Nuclear counter stain: DAPI

    Comments: Confocal image showing negative staining on NIH/3T3 cells

    Target primary antibody dilution: 1:500

    Target secondary antibody dilution: 1:1000 (2ug/mL)

    Counterstain primary antibody dilution: 1:1000 (1ug/mL)

    Counterstain secondary antibody dilution: 1:1000 (2ug/mL)

    Negative control 1 primary antibody dilution: 1:500 (Ab97959)

    Negative control 1 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150120)

    Negative control 2 primary antibody dilution: 1:1000 (1ug/mL) (Ab7291)

    Negative control 2 secondary antibody dilution: 1:1000 (2ug/mL) (Ab150077)

Anti-SOX2 antibody (ab97959)参考文献

This product has been referenced in:
  • Hornsby AK  et al. Short-term calorie restriction enhances adult hippocampal neurogenesis and remote fear memory in a Ghsr-dependent manner. Psychoneuroendocrinology 63:198-207 (2016). IHC-FrFl ; Mouse . Read more (PubMed: 26460782) »
  • Hellner K  et al. Premalignant SOX2 overexpression in the fallopian tubes of ovarian cancer patients: Discovery and validation studies. EBioMedicine 10:137-49 (2016). ICC/IF ; Chicken . Read more (PubMed: 27492892) »

See all 51 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human iPSC cells)
Permeabilization Yes - 0.5% TX100
Specification human iPSC cells
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Oct 10 2016

Application Immunocytochemistry
Sample Mouse Cell (mouse neural stem cell)
Permeabilization No
Specification mouse neural stem cell
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Ms. kyungjoo seong

Verified customer

提交于 Sep 22 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (lungs)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6, vector labs
Specification lungs
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 May 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (iPSC)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification iPSC
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 08 2016

Application IHC - Wholemount
Sample Xenopus laevis Embryo (embryo stage 34)
Specification embryo stage 34
Username

Abcam user community

Verified customer

提交于 Oct 29 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Antigen retrieval step Heat mediated
Sample Xenopus laevis Tissue sections (otic vesicle)
Specification otic vesicle
Permeabilization Yes - 0.4% Triton X in 3% normal horse serum
Fixative Bouin's
Username

Bharti Katbamna

Verified customer

提交于 Oct 27 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Mouse Tissue lysate - whole (1-wk-old mouse cerebellum)
Specification 1-wk-old mouse cerebellum
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 03 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4 · Temperature: 20°C
Antigen retrieval step None
Sample Mouse Tissue sections (brain with human tumor)
Specification brain with human tumor
Permeabilization No
Fixative Paraformaldehyde
Username

Ms. Francoise Geffroy

Verified customer

提交于 Apr 21 2014

Application Immunoprecipitation
Immuno-precipitation step Other - covalently coupled to protein G beads
Sample Mouse Cell lysate - whole cell (Embryonic stem cells)
Specification Embryonic stem cells
Treatment DMSO or GlcNAcstatin C (Oga inhibitor) for 24h
Total protein in input 100 µg
Username

Abcam user community

Verified customer

提交于 Apr 04 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Sample (Paddlefish) Polyodon spathula Tissue sections (otic vesicle)
Specification otic vesicle
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 04 2014

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