The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Contains 1 HMG box DNA-binding domain.
Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
All lanes : Anti-SOX2 antibody (ab75627) at 1/50 dilution
Lane 1 : 293 cell lysates nontransfected Lane 2 : 293 cell lysates transiently transfected with the SOX2 gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 34 kDa Observed band size : 34 kDa
Immunocytochemistry - SOX2 antibody (ab75627)This image is courtesy of an Abreview submitted by Parinya Noisa
ab75627 staining SOX2 from human neural stem cells derived from hESCscells by immunocytochemistry. Cells were paraformaldehyde fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 18 hours at 4°C. An Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/500, was used as the secondary antibody. Left image shows nuclei staining with Dapi (blue) and right shows ab75627 staining (green).