概述

  • 产品名称Anti-SOX2抗体
    参阅全部 SOX2 一抗
  • 描述
    兔多克隆抗体to SOX2
  • 经测试应用适用于: ICC, WB, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Sheep, Chicken, Xenopus laevis, Zebrafish, a wide range of other species
  • 免疫原

    Synthetic peptide:

    KEHPDYKYRPRRKTK

    , corresponding to amino acids 103-117 of Human SOX2. This sequence is identical to amino acids 113-127 of Human SOX1.

  • 阳性对照
    • Normal human brain lysate.

应用

Our Abpromise guarantee covers the use of ab69893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent concentration. See Abreview.
WB 1/500. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Flow Cyt Use at an assay dependent concentration. PubMed: 22741033ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • 疾病相关Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • 序列相似性Contains 1 HMG box DNA-binding domain.
  • 翻译后修饰Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Anti-SOX2 antibody 图像

  • Anti-SOX2 antibody (ab69893) at 1/500 dilution + normal human brain lysate at 10 µg

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

Anti-SOX2 antibody (ab69893)参考文献

This product has been referenced in:
  • Donovan LK  et al. A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by Oxygen Tension-Modulated CD133 Expression but Not Accompanied by Underlying Molecular Profiles. Transl Oncol 5:141-54 (2012). ICC/IF, Flow Cyt ; Human . Read more (PubMed: 22741033) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (Neural stem cells derived from hESCs)
Specification Neural stem cells derived from hESCs
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Parinya Noisa

Verified customer

提交于 Oct 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"