概述

  • 产品名称Anti-SOX2抗体
    参阅全部 SOX2 一抗
  • 描述
    山羊多克隆抗体to SOX2
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human, Apteronotus leptorhynchus
  • 免疫原

    Synthetic peptide from Human SOX2 (UniProt ID: P48431).

  • 阳性对照
    • Human brain (cerebellum) and testis tissues

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab110145 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P Use a concentration of 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • 疾病相关Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • 序列相似性Contains 1 HMG box DNA-binding domain.
  • 翻译后修饰Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Anti-SOX2 antibody 图像

  • ab110145, at 3.75 µg/ml, staining SOX2 in formalin fixed, paraffin embedded Human brain (cerebellum) by Immunohistochemistry.
  • ab110145, at 3.75 µg/ml, staining SOX2 in formalin fixed, paraffin embedded Human testis by Immunohistochemistry.

Anti-SOX2 antibody (ab110145)参考文献

This product has been referenced in:
  • Pakan JM & McDermott KW A method to investigate radial glia cell behavior using two-photon time-lapse microscopy in an ex vivo model of spinal cord development. Front Neuroanat 8:22 (2014). IHC (PFA fixed) . Read more (PubMed: 24782718) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Human Tissue sections (human tumor in mouse brain)
Antigen retrieval step None
Permeabilization Yes - triton 0.2%
Specification human tumor in mouse brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative Paraformaldehyde
Username

Ms. Francoise Geffroy

Verified customer

提交于 Apr 20 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (Blastocyst (D7 after IVF))
Specification Blastocyst (D7 after IVF)
Fixative Formaldehyde
Permeabilization Yes - 1% Triton X-100 (30 min)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

提交于 Mar 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Jan 28 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 May 12 2012

Vielen Dank fürIhre Anfrage.

Leider haben wirH00006657-A01 derzeit nicht in unserem Katalog verfügbar. Allerdings kann ich Ihnen gerne drei unserer anti-SOX2- Antikörper empfehlen, die wir für dieImmunhistochemie an humane...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"