概述

  • 产品名称
    Anti-SOX15抗体
    参阅全部 SOX15 一抗
  • 描述
    兔多克隆抗体to SOX15
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 180-229 (SHCKLEAPSP CSLPQSDPRL QGELLPTYTH YLPPGSPTPY NPPLAGAPMP) of human SOX15 (NP_008873).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab82921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

靶标

  • 功能
    Binds to the 5'-AACAAT-3' sequence.
  • 组织特异性
    Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.
  • 序列相似性
    Contains 1 HMG box DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • Protein SOX-12 antibody
      • Protein SOX-15 antibody
      • Protein SOX-20 antibody
      • SOX 12 protein antibody
      • SOX 15 antibody
      • SOX 15 protein antibody
      • SOX 20 protein antibody
      • SOX12 antibody
      • SOX12 protein antibody
      • SOX15 antibody
      • SOX15 protein antibody
      • SOX15_HUMAN antibody
      • SOX20 antibody
      • SOX20 protein antibody
      • SOX26 antibody
      • SOX27 antibody
      • SRY box 15 antibody
      • SRY sex determining region Y box 15 antibody
      • SRY sex determining region Y box 20 antibody
      • SRYbox 15 antibody
      see all

    图片

    • Western blot - SOX15 antibody (ab82921)
      Western blot - SOX15 antibody (ab82921)
      Anti-SOX15 antibody (ab82921) at 1 µg/ml + Transfected 293T cell lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa
      Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

    实验方案

    文献

    ab82921 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab82921？请让我们知道，以便我们可以引用本数据表中的参考文章。

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