概述

  • 产品名称Anti-SOX15抗体
    参阅全部 SOX15 一抗
  • 描述
    小鼠单克隆抗体to SOX15
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human SOX15 protein (AAH00985) with a 26 kDa tag.

  • 阳性对照
    • Hela nuclear extract; immunogen

性能

应用

Our Abpromise guarantee covers the use of ab77056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Binds to the 5'-AACAAT-3' sequence.
  • 组织特异性Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.
  • 序列相似性Contains 1 HMG box DNA-binding domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Protein SOX-12 antibody
    • Protein SOX-15 antibody
    • Protein SOX-20 antibody
    • SOX 12 protein antibody
    • SOX 15 antibody
    • SOX 15 protein antibody
    • SOX 20 protein antibody
    • SOX12 antibody
    • SOX12 protein antibody
    • SOX15 antibody
    • SOX15 protein antibody
    • SOX15_HUMAN antibody
    • SOX20 antibody
    • SOX20 protein antibody
    • SOX26 antibody
    • SOX27 antibody
    • SRY box 15 antibody
    • SRY sex determining region Y box 15 antibody
    • SRY sex determining region Y box 20 antibody
    • SRYbox 15 antibody
    see all

Anti-SOX15 antibody 图像

  • Anti-SOX15 antibody (ab77056) at 1/500 dilution + recombinant tagged SOX15 protein (the immunogen) at 0.2 µg

    Secondary
    goat anti-mouse IgG (H&L)- HRP conjugate at 1/5000 dilution

    Predicted band size : 25 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  • Anti-SOX15 antibody (ab77056) at 1/500 dilution + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)

Anti-SOX15 antibody (ab77056)参考文献

This product has been referenced in:
  • Pacini S  et al. Constitutive expression of pluripotency-associated genes in mesodermal progenitor cells (MPCs). PLoS One 5:e9861 (2010). WB, ICC/IF ; Human . Read more (PubMed: 20360837) »

See 1 Publication for this product

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