概述

  • 产品名称
    Anti-SOX14抗体
  • 描述
    兔多克隆抗体to SOX14
  • 特异性
    This antibody is specific for SOX14
  • 经测试应用
    适用于: ELISA, WB, ICCmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Dog
  • 免疫原

    A region within synthetic peptide: SSSAIQKMGE VPHTLATGAL PYASTLGYQN GAFGSLSCPS QHTHTHPSPT, corresponding to internal sequence amino acids 145-194 of Human SOX14

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab49047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/62500.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC Use at an assay dependent concentration. PubMed: 24637840

靶标

  • 相关性
    The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate (specifically neuronal). SOX14 is most closely related to SOX factors 1,2 and 3 and likely mirrors expression of these genes in the developing brain and spinal cord.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • HMG box transcription factor SOX 14 antibody
    • MGC119898 antibody
    • MGC119899 antibody
    • SOX 14 antibody
    • SOX 28 antibody
    • Sox box protein 14 antibody
    • SOX28 antibody
    • SRY (sex determining region Y) box 14 antibody
    • SRY box 14 antibody
    • Transcription factor SOX 14 antibody
    see all

图片

  • Anti-SOX14 antibody (ab49047) at 2.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 26 kDa
    Observed band size : 26 kDa

文献

This product has been referenced in:
  • Li F  et al. SOX14 promotes proliferation and invasion of cervical cancer cells through Wnt/ß-catenin pathway. Int J Clin Exp Pathol 8:1698-704 (2015). Read more (PubMed: 25973056) »
  • Popovic J  et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014). WB, ICC ; Human . Read more (PubMed: 24637840) »

See all 3 Publications for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Formaldehyde
Permeabilization
Yes - PBS-T
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 18 2012

Thank you for your reply.

As I mentioned in my previous email, my colleague has already issued you discount codes for both ab18529 & ab49047 and they are still valid until November 2012, therefore we do not need to issue new discount codes...

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Thank you for your reply.

According to our notes, you were issued 3 discount codes, one for ab18259, ab23470 & ab49047, to test each one in IF on chick samples.

Which antibody is it that you are no longer testing and also what is ...

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Thank you for contacting Abcam.

The Testing discount codes that my colleague issued to you in the *are valid until the *, therefore you can still use those discount codes.

Please let me know if there is anything else I can help you with.

I am very pleased to hear you would like to accept our offer and test these products in IF on chick. Each code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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