重组
RabMAb

Anti-SOX1抗体[EPR4766] (ab109290)

概述

  • 产品名称
    Anti-SOX1抗体[EPR4766]
    参阅全部 SOX1 一抗
  • 描述
    兔单克隆抗体[EPR4766] to SOX1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human SOX1.

  • 阳性对照
    • NCCIT, SH-SY5Y, MCF7, and HeLa cell lysates, Human fetal brain tissue, NCCIT cells
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab109290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • 应用说明
    Is unsuitable for IP.
  • 靶标

    图片

    • All lanes : Anti-SOX1 antibody [EPR4766] (ab109290) at 1/1000 dilution

      Lane 1 : NCCIT cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    • Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue using ab109290 at a dilution of 1/100.
    • Immunofluorescent staining of NCCIT cells using ab109290 at a dilution of 1/100.
    • Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling SOX1 with unpurified ab109290 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    文献

    This product has been referenced in:
    • Topalovic V  et al. Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells. PLoS One 12:e0184099 (2017). Read more (PubMed: 28886103) »
    • Popovic J  et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014). WB ; Human . Read more (PubMed: 24637840) »

    See all 4 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (ES cells)
    Permeabilization
    Yes - 0.1% PBST
    Specification
    ES cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Dec 08 2016

    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (E8.5)
    Specification
    E8.5
    Username

    Abcam user community

    Verified customer

    提交于 Dec 08 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain e14)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain e14
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Dec 02 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E10.5 mouse embryo)
    Permeabilization
    Yes - PBS/0.1% Triton X-100
    Specification
    E10.5 mouse embryo
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: R.T°C
    Fixative
    Formaldehyde
    Username

    Dr. Anestis Tsakiridis

    Verified customer

    提交于 Oct 30 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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