概述

  • 产品名称Anti-SOS2抗体
    参阅全部 SOS2 一抗
  • 描述
    兔多克隆抗体to SOS2
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Gorilla, Chinese Hamster, Orangutan, Platypus (Ornithorhynchus anatinus), Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1282 -1332 of Human SOS2 (NP_008870.2)

  • 阳性对照
    • HeLa and 293T whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab85831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 153 kDa.
IP Use at 2-5 µg/mg of lysate.

靶标

Anti-SOS2 antibody 图像

  • All lanes : Anti-SOS2 antibody (ab85831) at 0.1 mg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 5 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 50 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 153 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Immunoprecipitation/ Western Blot of ab85831
    Lane 1: ab85831 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab85831 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 30 seconds.

Anti-SOS2 antibody (ab85831)参考文献

ab85831 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"