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Synthetic peptide conjugated to KLH derived from within residues 2150 to the C-terminus of Human SorLA.
(Peptide available as ab16691.)
Our Abpromise guarantee covers the use of ab16642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC (PFA fixed)||Use a concentration of 0.6 - 1 µg/ml.|
Immunofluorescent staining for SorLA in rat cortical neurons using Rabbit polyclonal to SorLA (ab16642). The staining is cytoplasmic and punctate in these cortical neurons.
Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab16642 was incubated overnight at 1/500 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.