概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to SORBS2
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length (aa 1-1100) human SORBS2 protein (NP_066547.1).

  • 阳性对照
    • HepG2 cells; SORBS2 transfected 293T cell lysate.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab73444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 or with AKT1 and PAK1, thus mediating AKT1-mediated activation of PAK1. May play a role in the regulation of pancreatic cell adhesion, possibly by acting on WASF1 phosphorylation, enhancing phosphorylation by ABL1, as well as dephosphorylation by PTPN12 (PubMed:18559503). Isoform 6 increases water and sodium absorption in the intestine and gall-bladder.
  • 组织特异性
    Abundantly expressed in heart. In cardiac muscle cells, located in the Z-disks of sarcomere. Also found, but to a lower extent, in small and large intestine, pancreas, thymus, colon, spleen, prostate, testis, brain, ovary and epithelial cells. In the pancreas, mainly expressed in acinar cells, duct cells and all cell types in islets (at protein level). Tends to be down-regulated in pancreatic adenocarcinomas ans metastases.
  • 序列相似性
    Contains 3 SH3 domains.
    Contains 1 SoHo domain.
  • 结构域
    The first 2 SH3 domains are required for WASF1-binding. All 3 SH3 domains can bind independently to PTPN12.
  • 翻译后修饰
    Ubiquitinated by CBL.
    Dephosphorylated by PTPN12.
  • 细胞定位
    Cytoplasm, perinuclear region. Apical cell membrane. Cell junction, focal adhesion. Cell projection, lamellipodium. Found at the Z-disk sarcomeres, stress fibers, dense bodies and focal adhesion. In pancreatic acinar cells, localized preferentially to the apical membrane. Colocalized with vinculin and filamentous actin at focal adhesions and lamellipodia of pancreatic cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Arg binding protein 2 antibody
    • Arg-binding protein 2 antibody
    • Arg/Abl interacting protein 2 antibody
    • Arg/Abl interacting protein antibody
    • Arg/Abl-interacting protein 2 antibody
    • ArgBP2 antibody
    • KIAA0777 antibody
    • PRO0618 antibody
    • sorbin and SH3 domain containing 2 antibody
    • Sorbin and SH3 domain-containing protein 2 antibody
    • Sorbin antibody
    • Sorbs2 antibody
    • SRBS2_HUMAN antibody
    see all

图片

  • ab73444 at 10µg/ml staining SORBS2 in HepG2 cells by Immunofluorescence.
  • All lanes : Anti-SORBS2 antibody (ab73444) at 1/500 dilution

    Lane 1 : SORBS2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 124 kDa
    Observed band size : 124 kDa

文献

This product has been referenced in:
  • Kawabori M  et al. Triggering receptor expressed on myeloid cells 2 (TREM2) deficiency attenuates phagocytic activities of microglia and exacerbates ischemic damage in experimental stroke. J Neurosci 35:3384-96 (2015). IHC-FoFr ; Mouse . Read more (PubMed: 25716838) »

See 1 Publication for this product

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