Anti-SORBS1抗体(ab4551)

概述

  • 产品名称Anti-SORBS1抗体
    参阅全部 SORBS1 一抗
  • 描述
    山羊多克隆抗体to SORBS1
  • 特异性This antibody is expected to recognise both human isoforms.
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide: GTFPGNYVKPLYL, corresponding to C terminal amino acids 804-816 of Human SORBS1.

  • 常规说明GenBank Accession Number – NP_006425; NP_056200. LocusLink ID - 10580 (human); 20411 (mouse). Gene Ontology terms - cytoskeletal protein binding; actin binding; cell-matrix adhesion; zonula adherens; cell-cell adherens junction.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab4551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Can be blocked with Human SORBS1 peptide (ab18388). No signal obtained yet but low background observed in human heart and 293 lysates at up to 2 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions.
  • 组织特异性Detected in skeletal muscle (at protein level). Widely expressed with highest levels in heart and skeletal muscle.
  • 序列相似性Contains 3 SH3 domains.
    Contains 1 SoHo domain.
  • 翻译后修饰O-glycosylated.
  • 细胞定位Cell junction > adherens junction. Cell membrane. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with actin stress fibers. Also detected at the plasma membrane and in neuronal intranuclear inclusions. Colocalized with PXN at focal adhesions during myogenic differentiation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c-Cbl-associated protein antibody
    • CAP antibody
    • CBL Associated Protein antibody
    • Ponsin antibody
    • SH3 domain protein 5 antibody
    • SH3D5 antibody
    • SH3P12 antibody
    • Sorbin and SH3 Domain Containing 1 antibody
    • Sorbin and SH3 domain-containing protein 1 antibody
    • Sorbs1 antibody
    • SRBS1_HUMAN antibody
    see all

Anti-SORBS1 antibody 图像

  • All lanes : Anti-SORBS1 antibody (ab4551)

    Lane 1 : Molecular weight marker (Bio-Rad Precision Plus Kaleidoscope standards)
    Lane 2 : 10 µl Human plasma 1 (600-800 µg protein)
    Lane 3 : 10 µl Human plasma 2 (600-800 µg protein)
    Lane 4 : 10 µl Human White Blood cells 1 (30-50 µg protein)
    Lane 5 : 10 µl Human White Blood cells 2 (30-50 µg protein)
    Lane 6 : 10 µl HEK293 whole cell lysate (prepared by scrapping 10 cm confluent dish into 4ml of 2x sample buffer (Sigma)

    Secondary
    Donkey anti-goat IgG (H+L) conjugated to Alexa-Fluor680 at 1/5000 dilution
  • ab4551 (2.5 µg/ml) staining SORBS1 in Human Skeletal Muscle by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows spotty staining on muscle fibres in longitudinal section.

Anti-SORBS1 antibody (ab4551)参考文献

ab4551 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (white blood cells)
Loading amount 40 µg
Specification white blood cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Aug 23 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"