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Our Abpromise guarantee covers the use of ab4551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Can be blocked with Human SORBS1 peptide (ab18388). No signal obtained yet but low background observed in human heart and 293 lysates at up to 2 µg/ml.|
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Image courtesy of a customer that wished to remain anonymous.
ab4551 (2.5 µg/ml) staining SORBS1 in Human Skeletal Muscle by IHC-P. Steamed antigen retrieval in citrate buffer pH6, AP-staining. This image shows spotty staining on muscle fibres in longitudinal section.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"