Anti-Sorbitol Dehydrogenase抗体(ab88509)

概述

  • 产品名称Anti-Sorbitol Dehydrogenase抗体
    参阅全部 Sorbitol Dehydrogenase 一抗
  • 描述
    小鼠多克隆抗体to Sorbitol Dehydrogenase
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human protein (NP_003095.1)

  • 阳性对照
    • Human liver or Jurkat cell lysate. Human prostate tissue.

性能

应用

Our Abpromise guarantee covers the use of ab88509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明IHC-P: Use at a concentration of 3 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
    • 组织特异性Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level).
    • 序列相似性Belongs to the zinc-containing alcohol dehydrogenase family.
    • 细胞定位Mitochondrion membrane. Cell projection > cilium > flagellum. Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DHSO_HUMAN antibody
      • L iditol 2 dehydrogenase antibody
      • L-iditol 2-dehydrogenase antibody
      • OTTHUMP00000161939 antibody
      • SDH antibody
      • Sorbitol dehydrogenase 1 antibody
      • Sorbitol dehydrogenase antibody
      • SORD 1 antibody
      • SORD antibody
      • SORD1 antibody
      see all

    Anti-Sorbitol Dehydrogenase antibody 图像

    • Anti-Sorbitol Dehydrogenase antibody (ab88509) at 1 µg/ml + Human liver lysate at 50 µg/ml

      Predicted band size : 38 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-Sorbitol Dehydrogenase antibody (ab88509) at 1 µg/ml + Jurkat cell lysate at 50 µg

      Predicted band size : 38 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Sorbitol Dehydrogenase antibody (ab88509) at 1 µg/ml

      Lane 1 : Transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 38 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Purified ab88509 at 3ug/ml staining Sorbitol Dehydrogenase in formalin-fixed, paraffin-embedded Human prostate tissue.

    Anti-Sorbitol Dehydrogenase antibody (ab88509)参考文献

    ab88509 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Wild-type Prostate)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
    Permeabilization No
    Specification Wild-type Prostate
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative Paraformaldehyde
    Username

    Dr. Lucy Penfold

    Verified customer

    提交于 Nov 09 2016

    Merci de nous avoir contactés.

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    Merci de nous avoir contactés.

    Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Je me permets de vous transférer le numéro de commande de remplacemen...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"