The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: No more than 1/64000.
WB: Use at a concentration of 0.1-1.0 µg/ml. Predicted molecular weight: 38 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level).
Belongs to the zinc-containing alcohol dehydrogenase family.
Mitochondrion membrane. Cell projection > cilium > flagellum. Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface.