The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
应用说明Is unsuitable for WB.
功能Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
组织特异性Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level).
序列相似性Belongs to the zinc-containing alcohol dehydrogenase family.
细胞定位Mitochondrion membrane. Cell projection > cilium > flagellum. Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface.
Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab123497 at 6 µg/mg lysate for IP. Subsequent Western blot detection used an antibody which recognized an downstream epitope of Sorbitol Dehydrogenase at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds