Anti-Sonic Hedgehog抗体[OTI10H6] (ab87382)


  • 产品名称Anti-Sonic Hedgehog抗体[OTI10H6]
    参阅全部 Sonic Hedgehog 一抗
  • 描述
    小鼠单克隆抗体[OTI10H6] to Sonic Hedgehog
  • 经测试应用适用于: WB, ICC/IF, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 24-197 of Human Sonic Hedgehog, expressed in E.coli

  • 阳性对照
    • WB: lysate from HEK293T cells transfected with Sonic Hedgehog IF: HeLa cells transiently transfected with Sonic Hedgehog
  • 常规说明

    The clone number has been updated from 10H6 to OTI10H6, both clone numbers name the same clone.


Our Abpromise guarantee covers the use of ab87382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 51 kDa.
ICC/IF Use at an assay dependent dilution.
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Sonic Hedgehog (ab19897). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to Sonic Hedgehog (ab19897) as Detection.


  • 功能Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
  • 组织特异性Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
  • 疾病相关Defects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
    Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
    Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
    Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression.
  • 序列相似性Belongs to the hedgehog family.
  • 翻译后修饰The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
    N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity.
  • 细胞定位Cell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell.
  • Information by UniProt
  • 数据库链接
  • 别名
    • HHG 1 antibody
    • HHG-1 antibody
    • HHG1 antibody
    • HLP 3 antibody
    • HLP3 antibody
    • Holoprosencephaly 3 antibody
    • HPE 3 antibody
    • HPE3 antibody
    • MCOPCB5 antibody
    • shh antibody
    • SHH_HUMAN antibody
    • SMMC I antibody
    • SMMCI antibody
    • Sonic Hedgehog (Drosophila) homolog antibody
    • sonic hedgehog homolog (Drosophila) antibody
    • Sonic hedgehog homolog antibody
    • Sonic hedgehog protein antibody
    • Sonic hedgehog protein C-product antibody
    • TPT antibody
    • TPTPS antibody
    see all

Anti-Sonic Hedgehog antibody [OTI10H6] 图像

  • Standard Curve for Sonic Hedgehog (Analyte: Sonic Hedgehog protein (Amino end active) (ab63216)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [10H6] to Sonic Hedgehog (ab87382) at 1µg/ml and Detector Antibody Rabbit polyclonal to Sonic Hedgehog (ab19897) at 0.5µg/ml.
  • All lanes : Anti-Sonic Hedgehog antibody [OTI10H6] (ab87382) at 1/2000 dilution

    Lane 1 : lysate from HEK293T cells transfected with control cDNA
    Lane 2 : lysate from HEK293T cells transfected with Sonic Hedgehog

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200169)


Anti-Sonic Hedgehog antibody [OTI10H6] (ab87382)参考文献

ab87382 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your call today, and I apologize that these antibodies still are not working.

As we discussed, I'm sending a free of charge ELISA kit ab100639 on the order ***, which I expect to arrive by Friday.

Please keep me update...

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Thank you for your call last week and for your patience while I have been in touch with my colleagues about these antibodies. I apologize for the delay getting back to you.

The capture antibody ab87382 was tested at a coating concentration of...

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