概述

  • 产品名称
    Anti-SON抗体
    参阅全部 SON 一抗
  • 描述
    兔多克隆抗体to SON
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence, corresponding to amino acids 1463-1598 of Human SON.

  • 阳性对照
    • RT4, U-251 MG cell lysates; Human small intestine tissue; U-251 MG cells.

性能

应用

Our Abpromise guarantee covers the use of ab121759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

靶标

  • 功能
    Represses hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. Might protect cells from apoptosis. Might be involved in pre-mRNA splicing.
  • 组织特异性
    Widely expressed, with the higher expression seen in leukocyte and heart.
  • 序列相似性
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 G-patch domain.
  • 结构域
    Contains 8 types of repeats which are distributed in 3 regions.
  • 细胞定位
    Nucleus speckle. Colocalizes with the pre-mRNA splicing factor SFRS2/SC-35.
  • Information by UniProt
  • 数据库链接
    • 别名
      • BASS1 antibody
      • Bax antagonist selected in saccharomyces 1 antibody
      • DBP-5 antibody
      • Negative regulatory element-binding protein antibody
      • NRE-binding protein antibody
      • Protein DBP-5 antibody
      • Protein SON antibody
      • SON antibody
      • SON protein antibody
      • SON_HUMAN antibody
      • SON3 antibody
      see all

    图片

    • Western blot - Anti-SON antibody (ab121759)
      Western blot - Anti-SON antibody (ab121759)
      All lanes : Anti-SON antibody (ab121759) at 1/250 dilution

      Lane 1 : RT4 cell lysate
      Lane 2 : U-251 MG cell lysate
      Lane 3 : Human Plasma lysate
      Lane 4 : Human Liver lysate
      Lane 5 : Human Tonsil lysate

      Developed using the ECL technique.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON antibody (ab121759)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON antibody (ab121759)
      ab121759 at 1/1200 dilution staining SON in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-SON antibody (ab121759)
      Immunocytochemistry/ Immunofluorescence - Anti-SON antibody (ab121759)
      ab121759, at 4 µg/ml, staining SON (green) in PFA-fixed, Triton X-100 permbabilized U-251 MG cells, by Immunofluorescence.

    文献

    ab121759 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab121759？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-SON antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293T)
    Permeabilization
    Yes - 0.1% Triton X100 in PBA
    Specification
    HEK293T
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
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    提交于 Mar 15 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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