概述

  • 产品名称Anti-SON抗体
    参阅全部 SON 一抗
  • 描述
    兔多克隆抗体to SON
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Pig, Chimpanzee, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 - 1000 of Human SON.

  • 阳性对照
    • This antibody gave a positive signal in Human Thymus tissue lysate as well as U937 and THP1 human whole cell lysates. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal duodenum.

性能

应用

Our Abpromise guarantee covers the use of ab109472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.

Can be used with methanol or paraformaldehyde-fixed cells.

IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 263 kDa (predicted molecular weight: 263 kDa).

靶标

  • 功能Represses hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. Might protect cells from apoptosis. Might be involved in pre-mRNA splicing.
  • 组织特异性Widely expressed, with the higher expression seen in leukocyte and heart.
  • 序列相似性Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 1 G-patch domain.
  • 结构域Contains 8 types of repeats which are distributed in 3 regions.
  • 细胞定位Nucleus speckle. Colocalizes with the pre-mRNA splicing factor SFRS2/SC-35.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BASS1 antibody
    • Bax antagonist selected in saccharomyces 1 antibody
    • DBP-5 antibody
    • Negative regulatory element-binding protein antibody
    • NRE-binding protein antibody
    • Protein DBP-5 antibody
    • Protein SON antibody
    • SON antibody
    • SON protein antibody
    • SON_HUMAN antibody
    • SON3 antibody
    see all

Anti-SON antibody 图像

  • ab109472(1/200) staining SON in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.05% TritonX100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

  • All lanes : Anti-SON antibody (ab109472) at 1 µg/ml

    Lane 1 : Human thymus tissue lysate - total protein (ab30146)
    Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
    Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 263 kDa
    Observed band size : 263 kDa
    Additional bands at : 100 kDa,270 kDa (possible post-translational modification),40 kDa,50 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109472 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
  • IHC image of SON staining in Human normal duodenum formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109472, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-SON antibody (ab109472)参考文献

ab109472 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.05% Triton X100 in PBS
Username

Dr. Kirk McManus

Verified customer

提交于 Apr 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"