Anti-Somatostatin Receptor 4抗体(ab49298)

概述

  • 产品名称Anti-Somatostatin Receptor 4抗体
    参阅全部 Somatostatin Receptor 4 一抗
  • 描述
    兔多克隆抗体to Somatostatin Receptor 4
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Pig
  • 免疫原

    A region within synthetic peptide: IAIFADTRPA RGGQAVACNL QWPHPAWSAV FVVYTFLLGF LLPVLAIGLC, corresponding to internal sequence amino acids 181-230 of Human Somatostatin Receptor 4

  • 阳性对照
    • Jurkat cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab49298 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

WB Use a concentration of 1.25 mg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Receptor for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. It is functionally coupled not only to inhibition of adenylate cyclase, but also to activation of both arachidonate release and mitogen-activated protein (MAP) kinase cascade. Mediates antiproliferative action of somatostatin in tumor cells.
  • 组织特异性Specifically expressed in fetal and adult brain, lung tissue, stomach, and in lesser quantities in the kidney, pituitary and adrenals.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Somatostatin receptor type 4 antibody
    • SS 4R antibody
    • SS-4-R antibody
    • SS4-R antibody
    • SS4R antibody
    • SSR4_HUMAN antibody
    • SST R4 antibody
    • SSTR 4 antibody
    • Sstr4 antibody
    see all

Anti-Somatostatin Receptor 4 antibody 图像

  • Lane 1 :
    Lane 2 : Anti-Somatostatin Receptor 4 antibody (ab49298) at 1.25 µg/ml

    Lane 1 : MW marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ICC/IF image of ab49298 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49298, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Somatostatin Receptor 4 antibody (ab49298)参考文献

ab49298 has not yet been referenced specifically in any publications.

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