Anti-Somatostatin Receptor 4抗体(ab28578)

概述

  • 产品名称
    Anti-Somatostatin Receptor 4抗体
    参阅全部 Somatostatin Receptor 4 一抗
  • 描述
    兔多克隆抗体to Somatostatin Receptor 4
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:

    (C)QQEALQPEPGRKRIPLTRTTTF

    , corresponding to C terminal amino acids 366-388 of Human Somatostatin Receptor 4

  • 阳性对照
    • ICC/IF: SKNSH cells.

性能

应用

Our Abpromise guarantee covers the use of ab28578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/2500.
WB 1/100 - 1/5000. Detects a band of approximately 40-50 kDa (predicted molecular weight: 42 kDa).
ICC/IF 1/10 - 1/200.

靶标

  • 功能
    Receptor for somatostatin-14. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. It is functionally coupled not only to inhibition of adenylate cyclase, but also to activation of both arachidonate release and mitogen-activated protein (MAP) kinase cascade. Mediates antiproliferative action of somatostatin in tumor cells.
  • 组织特异性
    Specifically expressed in fetal and adult brain, lung tissue, stomach, and in lesser quantities in the kidney, pituitary and adrenals.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Somatostatin receptor type 4 antibody
    • SS 4R antibody
    • SS-4-R antibody
    • SS4-R antibody
    • SS4R antibody
    • SSR4_HUMAN antibody
    • SST R4 antibody
    • SSTR 4 antibody
    • Sstr4 antibody
    see all

图片

  • Immunofluorescent analysis of Somatostatin Receptor 4 (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Somatostatin Receptor 4 polyclonal antibody ab28578) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunofluorescent analysis of Somatostatin Receptor 4 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Somatostatin Receptor 4 polyclonal antibody (ab285781) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • ICC/IF image of ab28578 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28578, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

文献

ab28578 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
NDS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0,3% Trion X100
Fixative
Paraformaldehyde
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提交于 Nov 25 2013

Thank you for contacting us. ab28578 has been documented in IHC-P applications with human pheochromocytoma and human gastric mucosa sections. SSTR4 has been reported to be expressed in human brain, gastrointestinal tract, pancreas, and prostate tis...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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