Anti-Somatostatin Receptor 2抗体(ab124144)

概述

  • 产品名称Anti-Somatostatin Receptor 2抗体
    参阅全部 Somatostatin Receptor 2 一抗
  • 描述
    兔多克隆抗体to Somatostatin Receptor 2
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Chinese Hamster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Somatostatin Receptor 2.

  • 阳性对照
    • This antibody gave a positive signal in Rat and Mouse Pancreas tissue lysates within WB and in Mouse Pancreas formalin fixed paraffin embedded tissue section within IHC-P.

性能

应用

Our Abpromise guarantee covers the use of ab124144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 41 kDa).

靶标

  • 功能Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels.
  • 组织特异性Cerebrum and kidney. In lesser amounts in jejunum, colon and liver.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Somatostatin receptor type 2 antibody
    • SRIF-1 antibody
    • SRIF1 antibody
    • SS-2-R antibody
    • SS2-R antibody
    • SS2R antibody
    • SSR2_HUMAN antibody
    • SST2 antibody
    • SSTR2 antibody
    see all

Anti-Somatostatin Receptor 2 antibody 图像

  • All lanes : Anti-Somatostatin Receptor 2 antibody (ab124144) at 1 µg/ml

    Lane 1 : Pancreas (Rat) Tissue Lysate
    Lane 2 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 6 minutes
  • IHC image of Somatostatin Receptor 2 staining in mouse normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab124144, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Somatostatin Receptor 2 antibody (ab124144)参考文献

ab124144 has not yet been referenced specifically in any publications.

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